Sergeyev O V, Bosh'ian R E, Barinsky I F
I.M. Sechenov First Moscow State Medical University (Sechenov University).
National Research Centre for Epidemiology and Microbiology named after the honorary academician N.F. Gamaleya.
Vopr Virusol. 2020 Jul 21;65(3):126-131. doi: 10.36233/0507-4088-2020-65-3-126-131.
RETRACTEDHerpes simplex viruses types 1 (HSV-1) and 2 (HSV-2) are among the most common viruses in the human population. The clinical manifestations of HSV infection vary widely, which necessitates reliable molecular methods for the timely diagnosis of herpes virus infection, as well as for detection of mutations in the genes responsible for drug resistance. PCR is often unable to detect HSV isolates with nucleotide substitutions at the primer binding site. Sanger sequencing of the whole genome reveals mutations mainly at the consensus level, which accumulate at advanced stages of viral infection. High-throughput sequencing (HTS, next generation sequencing) offers an obvious advantage both in early diagnosis of herpes virus infection and identification of HSV variants.
撤回
单纯疱疹病毒1型（HSV-1）和2型（HSV-2）是人类中最常见的病毒之一。HSV感染的临床表现差异很大，这就需要可靠的分子方法来及时诊断疱疹病毒感染，以及检测负责耐药性的基因突变。聚合酶链反应（PCR）通常无法检测到在引物结合位点有核苷酸取代的HSV分离株。全基因组的桑格测序主要揭示了在病毒感染晚期积累的共识水平上的突变。高通量测序（HTS，下一代测序）在疱疹病毒感染的早期诊断和HSV变体的鉴定方面都具有明显优势。
