Marchenko V A, Barashkova S V, Zelinskaya I A, Toropova Y G, Sorokin E V, Zhilinskaya I N
Smorodintsev Research Institute of Influenza.
Almazov National Medical Research Centre.
Vopr Virusol. 2020 Jul 22;65(3):159-166. doi: 10.36233/0507-4088-2020-65-3-159-166.
It has now been established that blood vessels are target for influenza, but the mechanism by which the influenza virus affects the cardiovascular system is unknown. The aim - adaptation of influenza virus A/St. Petersburg/48/16 H1N1(pdm09) to mature Wistar rats, as these animals are the main experimental model for studying the pathology of the cardiovascular system.
Passage of influenza A virus (IAV) in embryonated chicken eggs, intranasal inoculation of rats with virus-containing material s, production of pulmonary homogenate, determination of IAV titer in embryonated chicken eggs, detection of histological changes in lung and pulmonary vessels.
The article presents the results of the adaptation of influenza virus A/St. Petersburg/48/16 H1N1(pdm09) to mature Wistar rats. The infectious titer of the virus in the homogenates of infected rats lungs at the last stage of adaptation was 7.0 lg EID50/ml. Histological studies revealed pronounced changes in the respiratory tract (spasm of bronchioles, submucosal edema, desquamation of ciliated epithelium of bronchioles) and pulmonary vessels (spasm, desquamation and swelling of endotheliocytes, dissociation and swelling of the elastic membrane and media). In order to identify IAV in blood vessels and lung tissues, an immunohistochemical study was performed using monoclonal antibodies to NP antigen of IAV.
The data obtained allow us to conclude that the strain of influenza virus A/St. Petersburg/48/16 H1N1(pdm09) was adapted to mature Wistar rats maintaining virulent properties. The infectious titer of the virus at the last stage of adaptation was 7.0 lg EID50/ml. IAV identification is confirmed by immunohistochemical examination.
现已确定血管是流感的靶标，但流感病毒影响心血管系统的机制尚不清楚。目的——将甲型流感病毒A/圣彼得堡/48/16 H1N1（pdm09）适应成年Wistar大鼠，因为这些动物是研究心血管系统病理学的主要实验模型。
甲型流感病毒（IAV）在鸡胚中传代，用含病毒材料经鼻接种大鼠，制备肺匀浆，测定鸡胚中IAV滴度，检测肺和肺血管的组织学变化。
本文介绍了甲型流感病毒A/圣彼得堡/48/16 H1N1（pdm09）适应成年Wistar大鼠的结果。适应最后阶段感染大鼠肺匀浆中病毒的感染滴度为7.0 lg EID50/ml。组织学研究显示呼吸道有明显变化（细支气管痉挛、粘膜下水肿、细支气管纤毛上皮脱落）和肺血管变化（痉挛、内皮细胞脱落和肿胀、弹性膜和中膜解离和肿胀）。为了在血管和肺组织中鉴定IAV，使用针对IAV NP抗原的单克隆抗体进行了免疫组织化学研究。
获得的数据使我们能够得出结论，甲型流感病毒A/圣彼得堡/48/16 H1N1（pdm09）毒株适应成年Wistar大鼠并保持毒力特性。适应最后阶段病毒的感染滴度为7.0 lg EID50/ml。免疫组织化学检查证实IAV鉴定结果。
