Functional Foods and Nutraceutical Unit, Department of Biochemistry, Federal University of Technology, Akure, Nigeria.
Department of Biomedical Technology, Federal University of Technology, Akure, Nigeria.
J Food Biochem. 2021 Feb;45(2):e13616. doi: 10.1111/jfbc.13616. Epub 2021 Feb 3.
This study sought to determine the life span promoting effecof orange (Citrus sinensis), tangerine (Citrus maxima) and grapefruit (Citrus paradisi) peels in Drosophila melanogaster model. Flies (both gender, 3 to 5 days old) were divided into seven (7) groups (n = 5) containing 40 flies each; group I (control) flies were fed with basal diet, II-VII were flies were fed with basal diet containing 0.1 and 1.0% of tangerine peel (TP), orange peel (CP), and grapefruit peel (GP) respectively, for 14 days. Locomotor performance and memory index were assessed via negative geotaxis and aversive phototaxis suppression assays, respectively. Thereafter, the fly homogenates were assayed for activities of acetylcholinesterase (AChE), monoamine oxidase (MAO) and antioxidant enzymes as well as other indices of their redox. The results revealed that the citrus peels significantly improved longevity, locomotor performance, memory index, antioxidant status, and modulate cholinesterase and monoamine oxidase enzyme activities in treated flies when compared to the control. The results obtained suggest that the citrus peels offer potentials as dietary supplement with life span promoting properties in D. melanogaster model which could as well serve as a functional food additives. PRACTICAL APPLICATIONS: Citrus peels, although often considered agro-wastes, are used as food supplements and food ingredents especially in production of candies, jams and custards. This study suggests the use of orange (Citrus sinensis), tangerine (Citrus maxima), and grapefruit (Citrus paradisi) peels as dietary supplements which offers potential life span promoting properties.
这项研究旨在确定橙子（Citrus sinensis）、橘子（Citrus maxima）和葡萄柚（Citrus paradisi）果皮在黑腹果蝇模型中的寿命促进作用。将果蝇（雌雄各半，3 至 5 天大）分为 7 组（n=5），每组含有 40 只果蝇；第 I 组（对照组）的果蝇喂食基础饮食，第 II-VII 组的果蝇喂食含有 0.1%和 1.0%的橘子皮（TP）、橙皮（CP）和葡萄柚皮（GP）的基础饮食，持续 14 天。通过负趋地性和厌恶光回避抑制试验评估运动表现和记忆指数。之后，测定果蝇匀浆中乙酰胆碱酯酶（AChE）、单胺氧化酶（MAO）和抗氧化酶的活性以及其他氧化还原指标。结果表明，与对照组相比，柑橘皮显著提高了寿命、运动表现、记忆指数、抗氧化状态，并调节了处理果蝇中的乙酰胆碱酯酶和单胺氧化酶的活性。研究结果表明，柑橘皮作为膳食补充剂具有延长寿命的潜力，可作为黑腹果蝇模型中的功能性食品添加剂。实际应用：尽管柑橘皮通常被认为是农业废物，但它们被用作食品补充剂和食品成分，特别是在糖果、果酱和奶油冻的生产中。本研究表明，使用橙子（Citrus sinensis）、橘子（Citrus maxima）和葡萄柚（Citrus paradisi）果皮作为膳食补充剂，具有潜在的延长寿命的特性。
