Gimeno I M, Shaw W N, Turner A, Bremen J, Cortes A L, Faiz N M, Gonder E, Robbins K
Department of Population Health and Pathobiology, Veterinary School, North Carolina State University, Raleigh, NC, USA.
Department of Veterinary Clinical Studies, Faculty of Veterinary Medicine, Universiti Putra Malaysia, Serdang, Malaysia.
Avian Pathol. 2021 Mar 17:1-7. doi: 10.1080/03079457.2021.1882937.
Understanding the pathogenesis of herpesvirus of turkeys (HVT) in its natural host is necessary before recombinant HVT (rHVT) can be used efficiently in turkey flocks. The objectives of this study were to evaluate when commercial turkey flocks get infected with wild type HVT, to study replication of HVT (conventional and recombinant rHVT-Newcastle disease, rHVT-ND) and other Marek's disease (MD) vaccines (SB-1 and CVI988) in turkey embryonic tissues, and to evaluate the expression of TLR-3 and IFN-γ in the lung and spleen of one-day-old turkeys after vaccination with MD vaccines. Our results demonstrated that commercial turkeys got exposed to wild type HVT within the first days of life; therefore, there is a potential of interaction between wild type HVT and rHVT when administered at day of age. On the other hand, all evaluated vaccines (especially HVT and rHVT-ND) replicated very well in turkey embryonic tissues. vaccination with HVT and CVI988 increased transcription of TLR-3 in the spleen of one-day-old turkeys. However, no effect on the transcription of TLR-3 or IFN-γ in the lungs and IFN-γ in the spleen in newly hatched turkeys was detected in the present study. Because of the limitations of evaluated genes, timepoints, and studied tissues, future studies are warranted to better understand the effect of MD vaccines on the turkey embryo immune responses. Commercial turkey flocks get infected with wild type HVT within the first days of life.HVT and rHVT replicates readily in turkey embryonic tissues.SB-1 and CVI988 also replicate in turkey embryonic tissues, but at lower rates than HVT and rHVT.HVT and CVI988 increase transcription of TLR-3 in the spleen.
在重组火鸡疱疹病毒（rHVT）能够在火鸡群中高效使用之前，了解其在天然宿主中的发病机制是必要的。本研究的目的是评估商业火鸡群何时感染野生型HVT，研究HVT（传统型和重组型rHVT - 新城疫，rHVT - ND）以及其他马立克氏病（MD）疫苗（SB - 1和CVI988）在火鸡胚胎组织中的复制情况，并评估在给1日龄火鸡接种MD疫苗后，肺和脾中Toll样受体3（TLR - 3）和干扰素γ（IFN - γ）的表达。我们的结果表明，商业火鸡在出生后的头几天内就接触到了野生型HVT；因此，在日龄时同时接种野生型HVT和rHVT存在相互作用的可能性。另一方面，所有评估的疫苗（尤其是HVT和rHVT - ND）在火鸡胚胎组织中复制良好。接种HVT和CVI988可增加1日龄火鸡脾脏中TLR - 3的转录。然而，在本研究中未检测到对新孵化火鸡肺中TLR - 3或IFN - γ以及脾中IFN - γ转录的影响。由于所评估的基因、时间点和研究组织存在局限性，有必要进行进一步研究以更好地了解MD疫苗对火鸡胚胎免疫反应的影响。商业火鸡群在出生后的头几天内感染野生型HVT。HVT和rHVT在火鸡胚胎组织中易于复制。SB - 1和CVI988也在火鸡胚胎组织中复制，但速率低于HVT和rHVT。HVT和CVI988增加脾脏中TLR - 3的转录。
