Department of Critical Care Medicine, Peking Union Medical College Hospital, Peking Union Medical College, Chinese Academy of Medical Sciences, Beijing, China.
Center of Clinical Laboratory, Zhongshan Hospital, Medical College of Xiamen University, Xiamen, China.
Shock. 2021 Sep 1;56(3):412-418. doi: 10.1097/SHK.0000000000001737.
To investigate the feasibility and the value of using mitochondrial quality control (MQC)-related proteins as biomarkers in septic patients.
The enrolled subjects were divided into four groups: healthy control group (n = 30), intensive care unit (ICU) control group (n = 62), septic nonshock group (n = 40), and septic shock group (n = 94). Serum levels of peroxisome proliferator-activated receptor γ coactivator 1α (PGC-1α), fission protein 1 (Fis1), mitofusin2 (Mfn2), and Parkin were measured by enzyme-linked immunosorbent assay at the time of enrollment for all groups. Clinical parameters and laboratory test results were also collected.
The levels of MQC-related biomarkers between any two of the four groups were significantly different (P < 0.001 for all). The serum levels of PGC-1α, Mfn2, and Parkin were lowest in healthy individuals; the levels were dramatically higher in the ICU control group compared with the others, and they decreased progressively from the septic nonshock group to the septic shock group. However, the pattern for Fis1 was inverse; the more severe the condition was, the higher the level of Fis1. Moreover, there was moderate correlation between MQC-related biomarkers and the SOFA score (PGC-1α, r = -0.662; Fis1, r = 0.609; Mfn2, r = -0.677; Parkin, r = 0.-0.674, P < 0.001 for all).
The serum levels of PGC-1α, Fis1, Mfn2, and Parkin were significantly correlated with organ dysfunction and reflected the disease progression and severity. The dynamic surveillance of these four biomarkers could be beneficial to predict outcome and guide treatment.
探讨线粒体质量控制（MQC）相关蛋白作为脓毒症患者生物标志物的可行性和价值。
纳入的研究对象分为四组：健康对照组（n = 30）、重症监护病房（ICU）对照组（n = 62）、非休克性脓毒症组（n = 40）和休克性脓毒症组（n = 94）。所有患者于入组时均采用酶联免疫吸附试验测定血清过氧化物酶体增殖物激活受体γ共激活因子 1α（PGC-1α）、分裂蛋白 1（Fis1）、融合蛋白 2（Mfn2）和 Parkin 的水平。同时收集临床参数和实验室检查结果。
四组间任何两组的 MQC 相关生物标志物水平均有显著差异（均 P < 0.001）。健康个体血清 PGC-1α、Mfn2 和 Parkin 水平最低；ICU 对照组水平显著高于其他三组，且从非休克性脓毒症组到休克性脓毒症组逐渐降低。而 Fis1 的模式则相反；病情越严重，Fis1 水平越高。此外，MQC 相关生物标志物与 SOFA 评分之间存在中度相关性（PGC-1α，r = -0.662；Fis1，r = 0.609；Mfn2，r = -0.677；Parkin，r = -0.674，均 P < 0.001）。
PGC-1α、Fis1、Mfn2 和 Parkin 血清水平与器官功能障碍显著相关，反映了疾病的进展和严重程度。这四种生物标志物的动态监测有助于预测预后和指导治疗。
