Department of Environmental Engineering, Technical University of Denmark, Bygningstorvet 115, 2800 Kongens Lyngby, Denmark.
Aquaporin A/S, Nymøllevej 78, 2800 Kongens Lyngby, Denmark.
Langmuir. 2021 Feb 16;37(6):2079-2090. doi: 10.1021/acs.langmuir.0c03044. Epub 2021 Feb 3.
Polymersomes made of amphiphilic diblock copolymers are generally regarded as having higher physical and chemical stability than liposomes composed of phospholipids. This enhanced stability arises from the higher molecular weight of polymer constituents. Despite their increased stability, polymer bilayers are solubilized by detergents in a similar manner to lipid bilayers. In this work, we evaluated the stability of poly(ethylene glycol)--poly(ε-caprolactone) (PEG-PCL)-based polymersomes exposed to three different detergents: -octyl-β-d-glucopyranoside (OG), lauryldimethylamine -oxide (LDAO), and Triton X-100 (TX-100). Changes in morphology, particle size distribution, and concentrations of the polymersomes were evaluated during the titration of the detergents into the polymersome solutions. Furthermore, we discussed the effect of detergent features on the solubilization of the polymeric bilayer and compared it to the results reported in the literature for liposomes and polymersomes. This information can be used for tuning the properties of PEG-PCL polymersomes for use in applications such as drug delivery or protein reconstitution studies.
由两亲性嵌段共聚物制成的聚合物囊泡通常被认为比由磷脂组成的脂质体具有更高的物理和化学稳定性。这种增强的稳定性源于聚合物成分的更高分子量。尽管聚合物双层的稳定性增加，但它们与脂质双层一样，可被去污剂溶解。在这项工作中，我们评估了暴露于三种不同去污剂的聚乙二醇-聚（ε-己内酯）（PEG-PCL）基聚合物囊泡的稳定性：辛基-β-D-吡喃葡萄糖苷（OG）、月桂基二甲基氧化胺（LDAO）和 Triton X-100（TX-100）。在将去污剂滴定到聚合物囊泡溶液中时，评估了聚合物囊泡的形态、粒径分布和浓度的变化。此外，我们讨论了去污剂特性对聚合物双层溶解的影响，并将其与文献中报道的脂质体和聚合物囊泡的结果进行了比较。这些信息可用于调整 PEG-PCL 聚合物囊泡的性质，以用于药物输送或蛋白质复性研究等应用。
