Dikomey E, Franzke J
Institut für Biophysik und Strahlenbiologie der Universität Hamburg, Federal Republic of Germany.
Radiat Environ Biophys. 1988;27(1):29-37. doi: 10.1007/BF01211107.
The kinetics of DNA denaturation in alkaline solution (pH 12.2) was studied in CHO cells using the alkaline unwinding technique. After X-ray doses of 0, 3, 5 and 9 Gy, the kinetics of alkaline denaturation was found to be independent of the number of induced strand breaks confirming earlier studies on this subject. In addition, the denaturation kinetics measured in cells exposed to 9 Gy were found to be identical for different repair intervals. This result shows that for the three different classes of DNA strand breaks described previously (Dikomey and Franzke 1986a) strand separation in alkaline solution occurs at the same kinetics. As a consequence, the relationship between the numbers of strand breaks and the fraction of remaining double-stranded DNA is considered the same for the three different classes.
使用碱性解旋技术，在CHO细胞中研究了碱性溶液（pH 12.2）中DNA变性的动力学。在0、3、5和9 Gy的X射线剂量后，发现碱性变性动力学与诱导链断裂的数量无关，这证实了此前关于该主题的研究。此外，发现在暴露于9 Gy的细胞中测量的变性动力学在不同的修复间隔下是相同的。该结果表明，对于先前描述的三种不同类型的DNA链断裂（Dikomey和Franzke，1986a），碱性溶液中的链分离以相同的动力学发生。因此，对于这三种不同类型，链断裂数量与剩余双链DNA比例之间的关系被认为是相同的。
