人源单克隆抗体对 E 蛋白结构域 III 的中和作用对寨卡病毒的影响。
Neutralization of Zika virus by E protein domain III-Specific human monoclonal antibody.
机构信息
Department of Biochemistry and Molecular Biology, Seoul National University College of Medicine, Seoul, 03080, Republic of Korea; Cancer Research Institute, Seoul National University College of Medicine, Seoul, 03080, Republic of Korea.
Department of Biochemistry and Molecular Biology, Seoul National University College of Medicine, Seoul, 03080, Republic of Korea; Department of Biomedical Science, Seoul National University College of Medicine, Seoul, 03080, Republic of Korea.
出版信息
Biochem Biophys Res Commun. 2021 Mar 19;545:33-39. doi: 10.1016/j.bbrc.2021.01.075. Epub 2021 Jan 31.
Zika virus (ZIKV) infection in both infants and adults is associated with neurological complications including, but not limited to, microcephaly and Guillain-Barre syndrome. Antibody therapy can be effective against virus infection. We isolated ZIKV envelope domain III-specific neutralizing antibodies (nAbs) from two convalescent patients with ZIKV infection. One antibody, 2F-8, exhibited potent in vitro neutralizing activity against Asian and American strains of ZIKV. To prevent FcγR-mediated antibody-dependent enhancement, we prepared IgG with LALA variation. A single dose of 2F-8 in the context of IgG or IgG-LALA prior to or post lethal ZIKV challenge conferred complete protection in mice.
寨卡病毒（ZIKV）感染可导致婴儿和成人出现神经并发症，包括但不限于小头畸形和格林-巴利综合征。抗体疗法对病毒感染可能有效。我们从两名寨卡病毒感染的恢复期患者中分离出了针对寨卡病毒包膜结构域 III 的中和抗体（nAb）。一种名为 2F-8 的抗体对亚洲和美洲株的寨卡病毒具有很强的体外中和活性。为了防止 FcγR 介导的抗体依赖增强作用，我们制备了具有 LALA 突变的 IgG。在致死性寨卡病毒攻击之前或之后，单次给予 IgG 或 IgG-LALA 中的 2F-8 可完全保护小鼠。
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