School of Chemistry and Biological Engineering, University of Science and Technology Beijing, Beijing 100083, China; Key Laboratory of Water and Sediment Sciences, Ministry of Education, College of Environmental Sciences and Engineering, Peking University, Beijing 100871, China; Department of Molecular Biosciences and Bioengineering, University of Hawaii at Manoa, Honolulu, HI 96822, USA.
School of Chemistry and Biological Engineering, University of Science and Technology Beijing, Beijing 100083, China.
Bioresour Technol. 2021 Apr;326:124699. doi: 10.1016/j.biortech.2021.124699. Epub 2021 Jan 12.
Utilization of glycerol, a biodiesel byproduct, has not been well explored. In the present study, glycerol and the other carbon sources were studied for cometabolism of dibenzothiophene (DBT), a model chemical commonly used in bioremediation studies, by Paraburkholderia sp. C3. This study showed a direct association between rhamnolipids (RLs) biosynthesis and DBT biodegradation induced by different carbon sources in a Paraburkholderia specie. Glycerol can induce the strain C3 produce at least four RLs. The RL precursor is mainly derived from the fatty acid synthesis (FAS II) and β-oxidation pathway. The genome contained two (fabF and fabG) and four (fadA, fadE, fadB and echA) genes involved in FAS II and β-oxidation, respectively. The genome also carried the rhlA and rhlB genes involved in rhamnosyltransferase for RL biosynthesis and two DBT dioxygenase genes (nahAc and catA). The findings suggest a viable approach of using the biodiesel byproduct glycerol to remediate contaminated environments.
甘油作为生物柴油的副产物，其利用尚未得到充分探索。本研究以模式污染物二苯并噻吩（DBT）为对象，研究了甘油与其他碳源在Paraburkholderia sp. C3 中的共代谢作用。该研究表明，在 Paraburkholderia 属中，不同碳源的生物降解与鼠李糖脂（RLs）的生物合成之间存在直接关联。甘油可以诱导菌株 C3 产生至少四种 RLs。RLs 的前体主要来源于脂肪酸合成（FAS II）和β-氧化途径。基因组包含两个（fabF 和 fabG）和四个（fadA、fadE、fadB 和 echA）分别参与 FAS II 和β-氧化的基因。基因组还携带参与 RL 生物合成的鼠李糖基转移酶的 rhlA 和 rhlB 基因以及两个 DBT 双加氧酶基因（nahAc 和 catA）。研究结果表明，利用生物柴油副产物甘油来修复污染环境是一种可行的方法。
