Pereira Evelyn Nunes Goulart da Silva, Silvares Raquel Rangel, Rodrigues Karine Lino, Flores Edgar Eduardo Ilaquita, Daliry Anissa
Laboratory of Cardiovascular Investigation, Oswaldo Cruz Institute, FIOCRUZ, Rio de Janeiro, Brazil.
Laboratory of Cardiovascular Investigation, Oswaldo Cruz Institute, FIOCRUZ, Rio de Janeiro, Brazil,
J Vasc Res. 2021 Feb 3:1-10. doi: 10.1159/000512832.
This study aims to examine the effect of a diet intervention and pyridoxamine (PM) supplementation on hepatic microcirculatory and metabolic dysfunction in nonalcoholic fatty liver disease (NAFLD).
NAFLD in Wistar rats was induced with a high-fat diet for 20 weeks (NAFLD 20 weeks), and control animals were fed with a standard diet. The NAFLD diet intervention group received the control diet between weeks 12 and 20 (NAFLD 12 weeks), while the NAFLD 12 weeks + PM group also received PM. Fasting blood glucose (FBG) levels, body weight (BW), visceral adipose tissue (VAT), and hepatic microvascular blood flow (HMBF) were evaluated at the end of the protocol.
The NAFLD group exhibited a significant increase in BW and VAT, which was prevented by the diet intervention, irrespective of PM treatment. The FBG was elevated in the NAFLD group, and caloric restriction improved this parameter, although additional improvement was achieved by PM. The NAFLD group displayed a 31% decrease in HMBF, which was partially prevented by caloric restriction and completely prevented when PM was added. HMBF was negatively correlated to BW, FBG, and VAT content.
PM supplementation in association with lifestyle modifications could be an effective intervention for metabolic and hepatic vascular complications.
本研究旨在探讨饮食干预和补充吡哆胺（PM）对非酒精性脂肪性肝病（NAFLD）肝脏微循环和代谢功能障碍的影响。
用高脂饮食诱导Wistar大鼠患NAFLD 20周（NAFLD 20周组），对照动物给予标准饮食。NAFLD饮食干预组在第12至20周期间给予对照饮食（NAFLD 12周组），而NAFLD 12周 + PM组还接受PM。在实验方案结束时评估空腹血糖（FBG）水平、体重（BW）、内脏脂肪组织（VAT）和肝脏微血管血流量（HMBF）。
NAFLD组的BW和VAT显著增加，饮食干预可预防这种增加，无论是否进行PM治疗。NAFLD组的FBG升高，热量限制改善了该参数，尽管PM可带来额外改善。NAFLD组的HMBF下降了31%，热量限制可部分预防，添加PM则可完全预防。HMBF与BW、FBG和VAT含量呈负相关。
补充PM并结合生活方式改变可能是对代谢和肝脏血管并发症的有效干预措施。
