限制酶。

Cold Spring Harb Protoc. 2021 Apr 1;2021(4):pdb.top101360. doi: 10.1101/pdb.top101360.

Restriction enzymes provided the foundation on which molecular cloning was built, and they remain as essential tools in current recombinant DNA technology. The three classes of restriction enzymes and their features are introduced here.

限制酶为分子克隆的构建提供了基础，它们仍然是当前重组 DNA 技术中不可或缺的工具。这里介绍了限制酶的三类及其特点。