Oh Youn-Lee, Choi In-Geol, Kong Won-Sik, Jang Kab-Yeul, Oh Min Ji, Im Ji-Hoon
Mushroom Science Division, National Institute of Horticultural and Herbal Science, Rural Development Administration, Eumseong, Korea.
Department of Biotechnology, College of Life Science and Biotechnology, Korea University, Seoul, Korea.
Mycobiology. 2020 Dec 17;49(1):61-68. doi: 10.1080/12298093.2020.1850172.
, commonly known as the button mushroom, is widely cultivated throughout the world. To breed new strains with more desirable traits and improved adaptability, diverse germplasm, including wild accessions, is a valuable genetic resource. To better understand the genetic diversity available in and identify previously unknown diversity within accessions, a phylogenetic analysis of 360 spp. accessions using single-nucleotide polymorphism genotyping was performed. Genetic relationships were compared using principal coordinate analysis (PCoA) among accessions with known origins and accessions with limited collection data. The accessions clustered into four groups based on the PCoA with regard to genetic relationships. A subset of 67 strains, which comprised a core collection where repetitive and uninformative accessions were not included, clustered into 7 groups following analysis. Two of the 170 accessions with limited collection data were identified as wild germplasm. The core collection allowed for the accurate analysis of genetic relationships, and accessions with an unknown pedigree were effectively grouped, allowing for origin identification, by PCoA analysis in this study.
平菇，通常被称为双孢蘑菇，在全球广泛种植。为培育具有更理想性状和更强适应性的新菌株，包括野生种质在内的多种种质是宝贵的遗传资源。为更好地了解平菇的遗传多样性并识别种质内先前未知的多样性，利用单核苷酸多态性基因分型对360个平菇属种质进行了系统发育分析。使用主坐标分析（PCoA）比较了已知起源的种质和收集数据有限的种质之间的遗传关系。根据PCoA分析，这些种质在遗传关系上聚类为四组。一个由67个菌株组成的子集，其中不包括重复和无信息的种质，在分析后聚类为7组。170个收集数据有限的种质中有两个被鉴定为野生种质。本研究中的核心种质集允许对平菇的遗传关系进行准确分析，并且通过PCoA分析有效地对系谱未知的种质进行了分组，从而实现了起源鉴定。
