• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

基于基因组分析一株引起菌血症性颈部脓肿的1049型高毒力菌株

Genome-Based Analysis of a Sequence Type 1049 Hypervirulent Causing Bacteremic Neck Abscess.

作者信息

Lan Peng, Zhao Dongdong, Gu Jiong, Shi Qiucheng, Yan Rushuang, Jiang Yan, Zhou Jiancang, Yu Yunsong

机构信息

Department of Infectious Diseases, Sir Run Run Shaw Hospital, Zhejiang University School of Medicine, Hangzhou, China.

Key Laboratory of Microbial Technology and Bioinformatics of Zhejiang Province, Hangzhou, China.

出版信息

Front Microbiol. 2021 Jan 18;11:617651. doi: 10.3389/fmicb.2020.617651. eCollection 2020.

DOI:10.3389/fmicb.2020.617651
PMID:33537016
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7848818/
Abstract

Hypervirulent (hvKP) has raised grave concerns in recent years and can cause severe infections with diverse anatomic locations including liver abscess, meningitis, and endophthalmitis. However, there is limited data about neck abscess caused by hvKP. A strain Kp_whw was isolated from neck abscess. We characterized the genetic background, virulence determinates of the strain by genomic analysis and dertermined the virulence level by serum resistance assay. Kp_whw belonged to sequence type (ST) 1049 K locus (KL) 5. Kp_whw showed hypermucoviscosity phenotype and was resistant to ampicillin but susceptible to the majority of the other antimicrobial agents. A pLVPK-like virulence plasmid and a chromosomal ICEKp5-like mobile genetic element were carried by Kp_whw, resulting in the risk of dissemination of hypervirulence. The strain exhibited relative higher level of core genome allelic diversity than accessory genome profile, in comparison to hvKP of K1/K2 serotype. Kp_whw was finally demonstrated as virulent as the ST23 K1 serotype hvKP strain NTUH-K2044 . In conclusion, this work elaborates the genetic background of a clinical hvKP strain with an uncommon ST, reinforcing our understanding of virulence mechanisms of hvKP.

摘要

近年来，高毒力肺炎克雷伯菌（hvKP）引发了严重关注，可导致包括肝脓肿、脑膜炎和眼内炎等不同解剖部位的严重感染。然而，关于hvKP引起颈部脓肿的数据有限。从颈部脓肿中分离出一株Kp_whw菌株。我们通过基因组分析对该菌株的遗传背景、毒力决定因素进行了表征，并通过血清抗性试验确定了其毒力水平。Kp_whw属于序列型（ST）1049、K位点（KL）5。Kp_whw表现出高黏液性表型，对氨苄西林耐药，但对大多数其他抗菌药物敏感。Kp_whw携带一个类似pLVPK的毒力质粒和一个类似染色体ICEKp5的可移动遗传元件，导致高毒力传播的风险。与K1/K2血清型的hvKP相比，该菌株核心基因组等位基因多样性水平相对高于辅助基因组图谱。最终证明Kp_whw与ST23 K1血清型hvKP菌株NTUH-K2044的毒力相当。总之，这项工作阐述了一株具有罕见ST的临床hvKP菌株的遗传背景，加深了我们对hvKP毒力机制的理解。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1220/7848818/adc871545a55/fmicb-11-617651-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1220/7848818/60725936eae1/fmicb-11-617651-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1220/7848818/2a866d5c6560/fmicb-11-617651-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1220/7848818/63cd408779c9/fmicb-11-617651-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1220/7848818/84f3cabf5398/fmicb-11-617651-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1220/7848818/f90c66658d48/fmicb-11-617651-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1220/7848818/0671b35cf9a3/fmicb-11-617651-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1220/7848818/adc871545a55/fmicb-11-617651-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1220/7848818/60725936eae1/fmicb-11-617651-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1220/7848818/2a866d5c6560/fmicb-11-617651-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1220/7848818/63cd408779c9/fmicb-11-617651-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1220/7848818/84f3cabf5398/fmicb-11-617651-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1220/7848818/f90c66658d48/fmicb-11-617651-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1220/7848818/0671b35cf9a3/fmicb-11-617651-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1220/7848818/adc871545a55/fmicb-11-617651-g007.jpg

相似文献

1
Genome-Based Analysis of a Sequence Type 1049 Hypervirulent Causing Bacteremic Neck Abscess.基于基因组分析一株引起菌血症性颈部脓肿的1049型高毒力菌株
Front Microbiol. 2021 Jan 18;11:617651. doi: 10.3389/fmicb.2020.617651. eCollection 2020.
2
Whole-Genome-Sequencing characterization of bloodstream infection-causing hypervirulent Klebsiella pneumoniae of capsular serotype K2 and ST374.全基因组测序分析导致血流感染的高毒力肺炎克雷伯菌 K2 荚膜血清型和 ST374 株的特征。
Virulence. 2018 Jan 1;9(1):510-521. doi: 10.1080/21505594.2017.1421894.
3
Clinical and Genomic Analysis of Liver Abscess-Causing Identifies New Liver Abscess-Associated Virulence Genes.导致肝脓肿的[具体病原体未给出]的临床与基因组分析确定了新的与肝脓肿相关的毒力基因。
Front Cell Infect Microbiol. 2016 Nov 29;6:165. doi: 10.3389/fcimb.2016.00165. eCollection 2016.
4
Core Genome Allelic Profiles of Clinical Klebsiella pneumoniae Strains Using a Random Forest Algorithm Based on Multilocus Sequence Typing Scheme for Hypervirulence Analysis.基于多位点序列分型方案的随机森林算法分析临床肺炎克雷伯菌菌株核心基因组等位基因谱的超毒力。
J Infect Dis. 2020 Mar 16;221(Suppl 2):S263-S271. doi: 10.1093/infdis/jiz562.
5
The Role of Plasmid and Resistance Gene Acquisition in the Emergence of ST23 Multi-Drug Resistant, Hypervirulent Klebsiella pneumoniae.质粒和耐药基因获得在 ST23 型多药耐药、高毒力肺炎克雷伯菌出现中的作用。
Microbiol Spectr. 2022 Apr 27;10(2):e0192921. doi: 10.1128/spectrum.01929-21. Epub 2022 Mar 17.
6
Virulence and genomic features of a and coharboring hypermucoviscous of serotype K2 and ST65.同时携带血清型K2和ST65高黏液性肺炎克雷伯菌的毒力和基因组特征
Infect Drug Resist. 2019 Jan 3;12:145-159. doi: 10.2147/IDR.S187289. eCollection 2019.
7
Characteristics of Hypervirulent : Does Low Expression of Contribute to the Absence of Hypervirulence?高毒力的特征：[具体基因名称]的低表达是否导致缺乏高毒力？ （注：原文中“Contribute to the Absence of Hypervirulence”前缺少具体基因相关内容，此处按推测补充了[具体基因名称]，以便更完整理解句子逻辑）
Front Microbiol. 2020 Mar 17;11:436. doi: 10.3389/fmicb.2020.00436. eCollection 2020.
8
Mapping the Evolution of Hypervirulent Klebsiella pneumoniae.绘制高毒力肺炎克雷伯菌的进化图谱。
mBio. 2015 Jul 21;6(4):e00630. doi: 10.1128/mBio.00630-15.
9
Diversity of virulence level phenotype of hypervirulent Klebsiella pneumoniae from different sequence type lineage.不同序列型系别的高毒力肺炎克雷伯菌毒力表型的多样性。
BMC Microbiol. 2018 Aug 29;18(1):94. doi: 10.1186/s12866-018-1236-2.
10
A fatal case of liver abscess caused by hypervirulent in a diabetic adolescent: A clinical and laboratory study.一名糖尿病青少年因高毒力菌株导致肝脓肿的致命病例：一项临床与实验室研究。
Pediatr Investig. 2021 Jan 26;5(2):118-124. doi: 10.1002/ped4.12238. eCollection 2021 Jun.

引用本文的文献

1
Antimicrobial resistance and molecular epidemiology of Klebsiella pneumoniae isolated from bovine mastitis in seven provinces in China.中国七个省份牛乳腺炎分离的肺炎克雷伯菌的耐药性及分子流行病学
BMC Microbiol. 2025 Jul 3;25(1):407. doi: 10.1186/s12866-025-04147-5.
2
Klebsiella pneumoniae under xylose pressure: the growth adaptation, antimicrobial susceptibility, global proteomics analysis and role of XylA and XylB proteins.木糖压力下的肺炎克雷伯菌：生长适应性、抗菌药敏性、全蛋白质组学分析以及木糖异构酶A和木糖异构酶B蛋白的作用
BMC Microbiol. 2025 Apr 29;25(1):257. doi: 10.1186/s12866-025-03961-1.
3
Prevalence of ST1049-KL5 carbapenem-resistant Klebsiella pneumoniae with a bla and bla co-carrying hypertransmissible IncM1 plasmid.

本文引用的文献

1
Core Genome Allelic Profiles of Clinical Klebsiella pneumoniae Strains Using a Random Forest Algorithm Based on Multilocus Sequence Typing Scheme for Hypervirulence Analysis.基于多位点序列分型方案的随机森林算法分析临床肺炎克雷伯菌菌株核心基因组等位基因谱的超毒力。
J Infect Dis. 2020 Mar 16;221(Suppl 2):S263-S271. doi: 10.1093/infdis/jiz562.
2
Comparison of core-genome MLST, coreSNP and PFGE methods for cluster analysis.比较核心基因组 MLST、核心 SNP 和 PFGE 方法用于聚类分析。
Microb Genom. 2020 Apr;6(4). doi: 10.1099/mgen.0.000347. Epub 2020 Mar 9.
3
Population genomics of Klebsiella pneumoniae.
ST1049-KL5 碳青霉烯类耐药肺炎克雷伯菌的流行，其携带 bla 和 bla 共同携带的超可传播 IncM1 质粒。
Commun Biol. 2024 Jun 6;7(1):695. doi: 10.1038/s42003-024-06398-w.
4
Recombinase-Aided Amplification Assay for Rapid Detection of Hypervirulent Klebsiella pneumoniae (hvKp) and Characterization of the hvKp Pathotype.用于快速检测高毒力肺炎克雷伯菌（hvKp）及鉴定hvKp致病型的重组酶辅助扩增检测法
Microbiol Spectr. 2023 Mar 13;11(2):e0398422. doi: 10.1128/spectrum.03984-22.
5
Development of a Novel Typing Scheme Based on the Genetic Diversity of Heme/Hemin Uptake System Hmu in Species Complex.基于血红素/高铁血红素摄取系统Hmu基因多样性的新型分型方案在物种复合体中的开发。
Microbiol Spectr. 2023 Feb 14;11(2):e0106222. doi: 10.1128/spectrum.01062-22.
6
Successful treatment of acute respiratory distress syndrome caused by hypervirulent with extracorporeal membrane oxygenation and continuous renal replacement therapy: A case report and literature review.采用体外膜肺氧合和持续肾脏替代疗法成功治疗高毒力引起的急性呼吸窘迫综合征：一例报告及文献综述。
Front Med (Lausanne). 2022 Aug 24;9:936927. doi: 10.3389/fmed.2022.936927. eCollection 2022.
7
Genomic and Immunological Characterization of Hypermucoviscous Carbapenem-Resistant ST25 Isolates from Northwest Argentina.阿根廷西北部高产黏液碳青霉烯类耐药 ST25 分离株的基因组和免疫学特征。
Int J Mol Sci. 2022 Jul 1;23(13):7361. doi: 10.3390/ijms23137361.
8
Bacterial Outer Membrane Permeability Increase Underlies the Bactericidal Effect of Fatty Acids From (Black Soldier Fly) Larvae Fat Against Hypermucoviscous Isolates of .细菌外膜通透性增加是黑水虻幼虫脂肪中脂肪酸对黏液高分泌型肺炎克雷伯菌分离株杀菌作用的基础。
Front Microbiol. 2022 May 6;13:844811. doi: 10.3389/fmicb.2022.844811. eCollection 2022.
肺炎克雷伯氏菌的群体基因组学。
Nat Rev Microbiol. 2020 Jun;18(6):344-359. doi: 10.1038/s41579-019-0315-1. Epub 2020 Feb 13.
4
Clinical, microbiological, and molecular epidemiological characteristics of -induced pyogenic liver abscess in southeastern China.中国东南部 - 引起的化脓性肝脓肿的临床、微生物学和分子流行病学特征。
Antimicrob Resist Infect Control. 2019 Oct 29;8:166. doi: 10.1186/s13756-019-0615-2. eCollection 2019.
5
A conjugative plasmid that augments virulence in Klebsiella pneumoniae.一种能增强肺炎克雷伯菌毒力的接合质粒。
Nat Microbiol. 2019 Dec;4(12):2039-2043. doi: 10.1038/s41564-019-0566-7. Epub 2019 Sep 30.
6
Clinical characteristics of bacteremia caused by hypermucoviscous Klebsiella pneumoniae at a tertiary hospital.三级医院高黏液性肺炎克雷伯菌菌血症的临床特征。
Diagn Microbiol Infect Dis. 2019 Sep;95(1):84-88. doi: 10.1016/j.diagmicrobio.2019.04.008. Epub 2019 Apr 26.
7
Hypervirulent Klebsiella pneumoniae.高毒力肺炎克雷伯菌。
Clin Microbiol Rev. 2019 May 15;32(3). doi: 10.1128/CMR.00001-19. Print 2019 Jun 19.
8
Monomicrobial gram-negative necrotizing fasciitis: An uncommon but fatal syndrome.单一细菌引起的革兰氏阴性坏死性筋膜炎：一种不常见但致命的综合征。
Diagn Microbiol Infect Dis. 2019 Jun;94(2):183-187. doi: 10.1016/j.diagmicrobio.2018.12.013. Epub 2019 Jan 4.
9
Genetic diversity, mobilisation and spread of the yersiniabactin-encoding mobile element ICEKp in Klebsiella pneumoniae populations.肠杆菌科细菌中携带耶尔森菌素的可移动元件 ICEKp 的遗传多样性、动员和传播。
Microb Genom. 2018 Sep;4(9). doi: 10.1099/mgen.0.000196. Epub 2018 Jul 9.
10
Hypervirulent Klebsiella pneumoniae, a 5-year study in a French ICU.高毒力肺炎克雷伯菌，法国 ICU 5 年研究。
J Med Microbiol. 2018 Aug;67(8):1083-1089. doi: 10.1099/jmm.0.000788. Epub 2018 Jul 4.