烟草花叶病毒的电场X射线散射测量

Electric field x-ray scattering measurements on tobacco mosaic virus.

作者信息

Koch M H, Dorrington E, Kläring R, Michon A M, Sayers Z, Marquet R, Houssier C

机构信息

European Molecular Biology Laboratory, c/o DESY, Hamburg, Federal Republic of Germany.

出版信息

Science. 1988 Apr 8;240(4849):194-6. doi: 10.1126/science.3353716.

DOI:10.1126/science.3353716

PMID:3353716

Abstract

The feasibility of electric field x-ray solution scattering with biological macromolecules was investigated. Electric field pulses (1.25 to 5.5 kilovolts per centimeter) were used to orient tobacco mosaic virus in solution (4.5 milligrams per milliliter). The x-ray scattering is characteristic of isolated oriented particles. The molecular orientation and its field-free decay were monitored with a time resolution of 2 milliseconds by means of synchrotron radiation and a multiwire proportional area detector. The method should also be applicable to synthetic polymers and inorganic colloids.

摘要

研究了电场X射线溶液散射用于生物大分子的可行性。使用电场脉冲（每厘米1.25至5.5千伏）使溶液中（每毫升4.5毫克）的烟草花叶病毒定向。X射线散射是孤立定向颗粒的特征。借助同步辐射和多丝正比面积探测器，以2毫秒的时间分辨率监测分子取向及其无场衰减。该方法也应适用于合成聚合物和无机胶体。