Ma Tianfang, Ungerleider Nathan, Zhang Derek Y, Corey Eva, Flemington Erik K, Dong Yan
Department of Structural and Cellular Biology, Tulane University School of Medicine, Tulane Cancer Center, New Orleans, LA, USA.
Department of Pathology, Tulane University School of Medicine, Tulane Cancer Center, New Orleans, LA, USA.
Data Brief. 2021 Jan 18;34:106774. doi: 10.1016/j.dib.2021.106774. eCollection 2021 Feb.
These data include secondary analysis of publicly available RNA-seq data from castration-resistant prostate cancer (CRPC) patients as well as RT-qPCR and Western blotting analyses of patient-derived xenograft models and a CRPC cell line. We applied Spearman correlation analysis to assess the relationship between canonical androgen receptor (AR) splicing and alternative AR splicing. We also assessed the ratio of AR splice variants (AR-Vs) to the full-length AR (AR-FL) at the RNA and protein levels by absolute RT-qPCR and Western blotting, respectively. These data are critical for studying the mechanisms underlying upregulated expression of AR-Vs after AR-directed therapies and the importance of AR-Vs to castration-resistant progression of prostate cancer. Data presented here are related to the research article by Ma et al., "Increased transcription and high translation efficiency lead to accumulation of androgen receptor splice variant after androgen deprivation therapy", Cancer Lett. In Press [1].
这些数据包括对去势抵抗性前列腺癌（CRPC）患者公开可用的RNA测序数据进行的二次分析，以及对患者来源的异种移植模型和一种CRPC细胞系进行的逆转录定量聚合酶链反应（RT-qPCR）和蛋白质免疫印迹分析。我们应用斯皮尔曼相关性分析来评估经典雄激素受体（AR）剪接与选择性AR剪接之间的关系。我们还分别通过绝对RT-qPCR和蛋白质免疫印迹在RNA和蛋白质水平评估了AR剪接变体（AR-Vs）与全长AR（AR-FL）的比例。这些数据对于研究AR导向治疗后AR-Vs表达上调的潜在机制以及AR-Vs对前列腺癌去势抵抗进展的重要性至关重要。此处呈现的数据与Ma等人发表于《癌症通讯》（Cancer Lett.）的研究文章“Increased transcription and high translation efficiency lead to accumulation of androgen receptor splice variant after androgen deprivation therapy”相关，该文章即将发表[1]。
