Central Laboratory, Seventh People's Hospital of Shanghai University of Traditional Chinese Medicine, Shanghai 200137, P.R. China.
Department of Nuclear Medicine, Seventh People's Hospital of Shanghai University of Traditional Chinese Medicine, Shanghai 200137, P.R. China.
Mol Med Rep. 2021 Apr;23(4). doi: 10.3892/mmr.2021.11885. Epub 2021 Feb 4.
Globally, thyroid cancer (TC) is considered to be the commonest endocrine malignancy. GINS complex subunit 2 (GINS2) belongs to the GINS complex family and is associated with cellular migration, invasion and growth. The present study aimed to investigate the underlying mechanisms of GINS2 on cell viability, migration and invasion in TC cells. By using MTT, wound healing and Transwell assays, the cell viability, migration and invasion were determined. Apoptosis was examined by immunofluorescence. Western blotting was used to detect protein expression levels. In the present study, biological function analysis demonstrated that GINS2 interference attenuated cell viability, migration and invasion in TC cell lines (K1 and SW579). It was discovered that, compared with the control group, GINS2 silencing induced apoptosis in TC cells. Additionally, GINS2 interference inhibited key proteins in the MAPK signaling pathway, including JNK, ERK and p38. According to these comparative experiments, GINS2 was considered to act a pivotal part in cell viability, migration and invasion of TC by regulating the MAPK signaling pathway and might be a potential therapeutic target for treating TC.
在全球范围内，甲状腺癌（TC）被认为是最常见的内分泌恶性肿瘤。GINS 复合物亚基 2（GINS2）属于 GINS 复合物家族，与细胞迁移、侵袭和生长有关。本研究旨在探讨 GINS2 在 TC 细胞中的细胞活力、迁移和侵袭中的潜在机制。通过 MTT、划痕愈合和 Transwell 检测法测定细胞活力、迁移和侵袭。通过免疫荧光法检测细胞凋亡。Western blot 检测蛋白表达水平。在本研究中，通过生物学功能分析表明，GINS2 干扰可减弱 TC 细胞系（K1 和 SW579）的细胞活力、迁移和侵袭。与对照组相比，GINS2 沉默可诱导 TC 细胞凋亡。此外，GINS2 干扰抑制 MAPK 信号通路中的关键蛋白，包括 JNK、ERK 和 p38。根据这些比较实验，GINS2 通过调节 MAPK 信号通路被认为在 TC 的细胞活力、迁移和侵袭中发挥关键作用，可能是治疗 TC 的潜在治疗靶点。
