• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

免疫 PET/NIRF/切伦科夫多模态成像在胰腺导管腺癌中细胞间黏附分子-1 的应用。

ImmunoPET/NIRF/Cerenkov multimodality imaging of ICAM-1 in pancreatic ductal adenocarcinoma.

机构信息

Department of Radiology, the First Affiliated Hospital of Xi'an Jiaotong University, 277 West Yanta Rd, Xi'an, 710061, Shaanxi, China.

Department of Radiology, University of Wisconsin-Madison, Room 7137, 1111 Highland Ave, Madison, WI, 53705, USA.

出版信息

Eur J Nucl Med Mol Imaging. 2021 Aug;48(9):2737-2748. doi: 10.1007/s00259-021-05216-3. Epub 2021 Feb 3.

DOI:10.1007/s00259-021-05216-3
PMID:33537836
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8266732/
Abstract

PURPOSE

We dual-labeled an intercellular adhesion molecule-1 (ICAM-1) monoclonal antibody (mAb) and evaluated its effectiveness for lesion detection and surgical navigation in pancreatic ductal adenocarcinoma (PDAC) via multiple noninvasive imaging approaches, including positron emission tomography (PET), near-infrared fluorescence (NIRF), and Cerenkov luminescence imaging (CLI).

METHODS

ICAM-1 expression in PDAC cell lines (BxPC-3 and AsPC-1) was assessed via flow cytometry and immunofluorescent staining. An ICAM-1 mAb labeled by IRDye 800CW and radionuclide zirconium-89 (denoted as [Zr]Zr-DFO-ICAM-1-IR800) was synthesized. Its performance was validated via in vivo comparative PET/NIRF/CLI and biodistribution (Bio-D) studies in nude mice bearing subcutaneous BxPC-3/AsPC-1 tumors or orthotopic BxPC-3 tumor models using nonspecific IgG as an isotype control tracer.

RESULTS

ICAM-1 expression was strong in the BxPC-3 and minimal in the AsPC-1 cell line. Both multimodality imaging and Bio-D data exhibited more prominent uptake of [Zr]Zr-DFO-ICAM-1-IR800 in BxPC-3 tumors than in AsPC-1 tumors. The uptake of [Zr]Zr-DFO-IgG-IR800 in BxPC-3 tumors was similar to that of [Zr]Zr-DFO-ICAM-1-IR800 in AsPC-1 tumors. These results demonstrate the desirable affinity and specificity of [Zr]Zr-DFO-ICAM-1-IR800 compared to [Zr]Zr-DFO-IgG-IR800. Orthotopic BxPC-3 tumor foci could also be clearly delineated by [Zr]Zr-DFO-ICAM-1-IR800. An intermodal match was achieved in the ICAM-1-targeted immunoPET/NIRF/CLI. The positive expression levels of ICAM-1 in BxPC-3 tumor tissue were further confirmed by immunohistopathology.

CONCLUSION

We successfully developed a dual-labeled ICAM-1-targeted tracer for PET/NIRF/CLI of PDAC that can facilitate better diagnosis and intervention of PDAC upon clinical translation.

摘要

目的

我们对细胞间黏附分子-1（ICAM-1）单克隆抗体（mAb）进行双标记，并通过多种非侵入性成像方法，包括正电子发射断层扫描（PET）、近红外荧光（NIRF）和切伦科夫发光成像（CLI），评估其在胰腺导管腺癌（PDAC）中的病灶检测和手术导航的有效性。

方法

通过流式细胞术和免疫荧光染色评估 PDAC 细胞系（BxPC-3 和 AsPC-1）中 ICAM-1 的表达。合成了一种通过 IRDye 800CW 和放射性核素锆-89 标记的 ICAM-1 mAb（表示为[Zr]Zr-DFO-ICAM-1-IR800）。通过在皮下接种 BxPC-3/AsPC-1 肿瘤或原位 BxPC-3 肿瘤模型的裸鼠中进行体内比较 PET/NIRF/Cli 和生物分布（Bio-D）研究，以非特异性 IgG 作为同种型对照示踪剂，验证其性能。

结果

ICAM-1 在 BxPC-3 细胞系中的表达较强，在 AsPC-1 细胞系中的表达较弱。多模态成像和 Bio-D 数据均显示，[Zr]Zr-DFO-ICAM-1-IR800 在 BxPC-3 肿瘤中的摄取明显高于 AsPC-1 肿瘤。BxPC-3 肿瘤中[Zr]Zr-DFO-IgG-IR800 的摄取与 AsPC-1 肿瘤中[Zr]Zr-DFO-ICAM-1-IR800 的摄取相似。这些结果表明，与[Zr]Zr-DFO-IgG-IR800 相比，[Zr]Zr-DFO-ICAM-1-IR800 具有理想的亲和力和特异性。[Zr]Zr-DFO-ICAM-1-IR800 还可以清晰地描绘出原位 BxPC-3 肿瘤灶。免疫 PET/NIRF/Cli 实现了模态间匹配。免疫组织病理学进一步证实了 BxPC-3 肿瘤组织中 ICAM-1 的阳性表达水平。

结论

我们成功开发了一种用于 PDAC 的双标记 ICAM-1 靶向示踪剂，可在临床转化后促进 PDAC 的更好诊断和干预。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3822/8266732/459fdadd7db2/nihms-1674417-f0006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3822/8266732/2a119f335dac/nihms-1674417-f0001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3822/8266732/a96e4d24ad63/nihms-1674417-f0002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3822/8266732/e1f723df0cf8/nihms-1674417-f0003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3822/8266732/713fae2ffaad/nihms-1674417-f0004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3822/8266732/f1f88c57167b/nihms-1674417-f0005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3822/8266732/459fdadd7db2/nihms-1674417-f0006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3822/8266732/2a119f335dac/nihms-1674417-f0001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3822/8266732/a96e4d24ad63/nihms-1674417-f0002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3822/8266732/e1f723df0cf8/nihms-1674417-f0003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3822/8266732/713fae2ffaad/nihms-1674417-f0004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3822/8266732/f1f88c57167b/nihms-1674417-f0005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3822/8266732/459fdadd7db2/nihms-1674417-f0006.jpg

相似文献

1
ImmunoPET/NIRF/Cerenkov multimodality imaging of ICAM-1 in pancreatic ductal adenocarcinoma.免疫 PET/NIRF/切伦科夫多模态成像在胰腺导管腺癌中细胞间黏附分子-1 的应用。
Eur J Nucl Med Mol Imaging. 2021 Aug;48(9):2737-2748. doi: 10.1007/s00259-021-05216-3. Epub 2021 Feb 3.
2
ImmunoPET of trophoblast cell-surface antigen 2 (Trop-2) expression in pancreatic cancer.免疫 PET 检测胰腺癌中滋养层细胞表面抗原 2（Trop-2）的表达。
Eur J Nucl Med Mol Imaging. 2022 Feb;49(3):861-870. doi: 10.1007/s00259-021-05563-1. Epub 2021 Sep 14.
3
Cadherin-17 as a target for the immunoPET of adenocarcinoma.黏附蛋白 17 作为腺癌免疫 PET 显像的靶点。
Eur J Nucl Med Mol Imaging. 2024 Jul;51(9):2547-2557. doi: 10.1007/s00259-024-06709-7. Epub 2024 Apr 16.
4
ImmunoPET Imaging of CD47 with VHH-Derived Tracers in Pancreatic Cancers.免疫 PET 成像技术应用 VHH 衍生示踪剂检测胰腺癌中的 CD47。
Mol Pharm. 2023 Aug 7;20(8):4184-4195. doi: 10.1021/acs.molpharmaceut.3c00311. Epub 2023 Jul 5.
5
Zr-PET imaging of DNA double-strand breaks for the early monitoring of response following α- and β-particle radioimmunotherapy in a mouse model of pancreatic ductal adenocarcinoma.Zr-PET 成像检测 DNA 双链断裂用于α和β粒子放射免疫治疗胰腺导管腺癌小鼠模型后的早期疗效监测。
Theranostics. 2020 Apr 27;10(13):5802-5814. doi: 10.7150/thno.44772. eCollection 2020.
6
Detecting TRA-1-60 in Cancer via a Novel Zr-89 Labeled ImmunoPET Imaging Agent.通过新型 Zr-89 标记免疫 PET 成像剂检测癌症中的 TRA-1-60。
Mol Pharm. 2020 Apr 6;17(4):1139-1147. doi: 10.1021/acs.molpharmaceut.9b01181. Epub 2020 Feb 28.
7
ImmunoPET Predicts Response to Met-targeted Radioligand Therapy in Models of Pancreatic Cancer Resistant to Met Kinase Inhibitors.免疫 PET 预测对转移性胰腺癌对 MET 激酶抑制剂耐药模型的放射性配体治疗的反应。
Theranostics. 2020 Jan 1;10(1):151-165. doi: 10.7150/thno.37098. eCollection 2020.
8
ImmunoPET Imaging of Insulin-Like Growth Factor 1 Receptor in a Subcutaneous Mouse Model of Pancreatic Cancer.胰岛素样生长因子1受体在胰腺癌皮下小鼠模型中的免疫正电子发射断层显像
Mol Pharm. 2016 Jun 6;13(6):1958-66. doi: 10.1021/acs.molpharmaceut.6b00132. Epub 2016 Apr 26.
9
Immuno-PET and Targeted α-Therapy Using Anti-Glypican-1 Antibody Labeled with Zr or At: A Theranostic Approach for Pancreatic Ductal Adenocarcinoma.免疫 PET 和使用 Zr 或 At 标记的抗 Glypican-1 抗体的靶向 α 治疗：用于胰腺导管腺癌的治疗方法。
J Nucl Med. 2023 Dec 1;64(12):1949-1955. doi: 10.2967/jnumed.123.266313.
10
ImmunoPET imaging of tissue factor expression in pancreatic cancer with Zr-Df-ALT-836.Zr-Df-ALT-836 用于胰腺癌组织因子表达的免疫 PET 成像。
J Control Release. 2017 Oct 28;264:160-168. doi: 10.1016/j.jconrel.2017.08.029. Epub 2017 Aug 24.

引用本文的文献

1
Advancements in molecular imaging for the diagnosis and treatment of pancreatic ductal adenocarcinoma.用于胰腺导管腺癌诊断和治疗的分子成像进展。
Nanoscale Adv. 2025 Apr 22;7(10):2887-2903. doi: 10.1039/d4na01080a. eCollection 2025 May 13.
2
Exploring the potential mechanisms of Da ChaiHu decoction against pancreatic cancer based on network pharmacology prediction and molecular docking approach.基于网络药理学预测和分子对接方法探索大柴胡汤抗胰腺癌的潜在机制
Naunyn Schmiedebergs Arch Pharmacol. 2025 Apr 23. doi: 10.1007/s00210-025-04107-w.
3
Comparison of Tumor Non-specific and PD-L1 Specific Imaging by Near-Infrared Fluorescence/Cerenkov Luminescence Dual-Modality In-situ Imaging.

本文引用的文献

1
Immuno-imaging of ICAM-1 in tumours by SPECT.通过单光子发射计算机断层扫描（SPECT）对肿瘤中细胞间黏附分子-1（ICAM-1）进行免疫成像。
Nucl Med Biol. 2020 May-Jun;84-85:73-79. doi: 10.1016/j.nucmedbio.2020.02.014. Epub 2020 Feb 25.
2
Immuno-PET imaging of VEGFR-2 expression in prostate cancer with Zr-labeled ramucirumab.用锆标记的雷莫西尤单抗对前列腺癌中VEGFR-2表达进行免疫正电子发射断层显像。
Am J Cancer Res. 2019 Sep 1;9(9):2037-2046. eCollection 2019.
3
Retooling a Blood-Based Biomarker: Phase I Assessment of the High-Affinity CA19-9 Antibody HuMab-5B1 for Immuno-PET Imaging of Pancreatic Cancer.
近红外荧光/切伦科夫光双模原位成像对肿瘤非特异性和 PD-L1 特异性成像的比较。
Mol Imaging. 2024 Jun 14;23:15353508241261473. doi: 10.1177/15353508241261473. eCollection 2024 Jan-Dec.
4
U-Net enhanced real-time LED-based photoacoustic imaging.基于 LED 的 U-Net 增强型实时光声成像。
J Biophotonics. 2024 Jun;17(6):e202300465. doi: 10.1002/jbio.202300465. Epub 2024 Apr 15.
5
Brachytherapy at the nanoscale with protein functionalized and intrinsically radiolabeled [Yb]YbO nanoseeds.用蛋白功能化和内源性放射性标记的 [Yb]YbO 纳米种子进行纳米尺度的近距离治疗。
Eur J Nucl Med Mol Imaging. 2024 May;51(6):1558-1573. doi: 10.1007/s00259-024-06612-1. Epub 2024 Jan 25.
6
Claudin18.2-targeted cancer theranostics.Claudin18.2靶向癌症诊疗一体化
Am J Nucl Med Mol Imaging. 2023 Apr 25;13(2):64-69. eCollection 2023.
7
In Vivo Evaluation of a Gallium-68-Labeled Tumor-Tracking Cyanine Dye for Positron Emission Tomography/Near-Infrared Fluorescence Carcinoma Imaging, Image-Guided Surgery, and Photothermal Therapy.用于正电子发射断层扫描/近红外荧光癌成像、图像引导手术和光热治疗的镓-68标记肿瘤追踪花青染料的体内评估
ACS Omega. 2023 Feb 3;8(6):6067-6077. doi: 10.1021/acsomega.2c08235. eCollection 2023 Feb 14.
8
Development and comparison of Ga/F/Cu-labeled nanobody tracers probing Claudin18.2.用于探测Claudin18.2的镓/氟/铜标记纳米抗体示踪剂的开发与比较
Mol Ther Oncolytics. 2022 Nov 14;27:305-314. doi: 10.1016/j.omto.2022.11.003. eCollection 2022 Dec 15.
9
A carbon monoxide releasing metal organic framework nanoplatform for synergistic treatment of triple-negative breast tumors.一种用于协同治疗三阴性乳腺癌的一氧化碳释放金属有机框架纳米平台。
J Nanobiotechnology. 2022 Nov 24;20(1):494. doi: 10.1186/s12951-022-01704-2.
10
ImmunoPET: Antibody-Based PET Imaging in Solid Tumors.免疫正电子发射断层显像：实体瘤中基于抗体的正电子发射断层显像成像
Front Med (Lausanne). 2022 Jun 28;9:916693. doi: 10.3389/fmed.2022.916693. eCollection 2022.
基于血液的生物标志物的再利用：高亲和力 CA19-9 抗体 HuMab-5B1 用于胰腺癌免疫 PET 成像的 I 期评估。
Clin Cancer Res. 2019 Dec 1;25(23):7014-7023. doi: 10.1158/1078-0432.CCR-18-3667. Epub 2019 Sep 20.
4
In Vivo Near-Infrared Fluorescence Imaging of Atherosclerosis Using Local Delivery of Novel Targeted Molecular Probes.利用新型靶向分子探针局部递送进行动脉粥样硬化的体内近红外荧光成像。
Sci Rep. 2019 Feb 25;9(1):2670. doi: 10.1038/s41598-019-38970-4.
5
Early Detection of Pancreatic Cancer: Opportunities and Challenges.早期胰腺癌检测：机遇与挑战。
Gastroenterology. 2019 May;156(7):2024-2040. doi: 10.1053/j.gastro.2019.01.259. Epub 2019 Feb 2.
6
Optical Imaging of Triple-Negative Breast Cancer Cells in Xenograft Athymic Mice Using an ICAM-1-Targeting Small-Molecule Probe.利用细胞间黏附分子-1（ICAM-1）靶向小分子探针对异种移植裸鼠中的三阴性乳腺癌细胞进行光学成像。
Mol Imaging Biol. 2019 Oct;21(5):835-841. doi: 10.1007/s11307-018-01312-3.
7
multiplex molecular imaging of vascular inflammation using surface-enhanced Raman spectroscopy.利用表面增强拉曼光谱对血管炎症进行多重分子成像。
Theranostics. 2018 Nov 29;8(22):6195-6209. doi: 10.7150/thno.28665. eCollection 2018.
8
Translational molecular imaging in exocrine pancreatic cancer.外分泌胰腺肿瘤的转化分子影像学
Eur J Nucl Med Mol Imaging. 2018 Dec;45(13):2442-2455. doi: 10.1007/s00259-018-4146-5. Epub 2018 Sep 17.
9
A novel plectin/integrin-targeted bispecific molecular probe for magnetic resonance/near-infrared imaging of pancreatic cancer.一种用于胰腺癌磁共振/近红外成像的新型桥联蛋白/整合素靶向双特异性分子探针。
Biomaterials. 2018 Nov;183:173-184. doi: 10.1016/j.biomaterials.2018.08.048. Epub 2018 Aug 26.
10
Soluble stroma-related biomarkers of pancreatic cancer.胰腺癌可溶性基质相关生物标志物。
EMBO Mol Med. 2018 Aug;10(8). doi: 10.15252/emmm.201708741.