Faculty of Health, University College Copenhagen, Copenhagen, Denmark.
Department of Clinical Immunology, Rigshospitalet, Copenhagen, Denmark.
Int J Lab Hematol. 2021 Oct;43(5):1207-1215. doi: 10.1111/ijlh.13470. Epub 2021 Feb 3.
Pneumatic tube systems (PTS) are frequently used for rapid and cost-effective transportation of blood samples to the clinical laboratory. The impact of PTS transport on platelet function measured by the Multiplate system and global hemostasis measured by the TEG 5000 was evaluated.
Paired samples from healthy adult individuals were obtained at two study sites: Rigshospitalet (RH) and Nordsjaellands Hospital (NOH). One sample was transported by PTS and one manually (non-PTS). Platelet function was assessed by platelet aggregation (Multiplate) and global hemostasis was assessed by a variety of thrombelastography (TEG) assays. Multiplate (n = 39) and TEG (n = 32) analysis was performed at site RH, and Multiplate (n = 28) analysis was performed at site NOH.
A significant higher agonist-induced platelet aggregation was found for PTS samples compared to manual transport at site NOH (P < .02, all agonists). No significant difference was found at site RH (P > .05, all agonists). For Kaolin TEG, samples transported by PTS showed a significant lower R-time and higher Angle (P < .001). No significant differences in MA and LY30 was found (P > .05). ACT of RapidTEG was significantly reduced (P = .001) and MA of Functional Fibrinogen TEG was significantly increased (P < .001) after PTS transport. No significant impact of PTS was observed for TEG assays with heparinase (P > .05).
Depending on the type of PTS, transportation by PTS affected platelet aggregation measured by Multiplate. Furthermore, PTS alters TEG parameters possibly reflecting coagulation factors. Clinical laboratories should evaluate the effect of the local PTS on Multiplate and TEG results.
气动输送系统（PTS）常用于快速且经济高效地将血液样本输送到临床实验室。本研究评估了 PTS 输送对 Multiplate 系统测量的血小板功能和 TEG 5000 测量的整体止血功能的影响。
在两个研究地点（Rigshospitalet [RH] 和 Nordsjaellands Hospital [NOH]）采集健康成年个体的配对样本。一份样本通过 PTS 输送，另一份通过手动（非 PTS）输送。通过血小板聚集（Multiplate）评估血小板功能，通过各种血栓弹力图（TEG）检测评估整体止血功能。在 RH 站点进行 Multiplate（n=39）和 TEG（n=32）分析，在 NOH 站点进行 Multiplate（n=28）分析。
与手动输送相比，NOH 站点 PTS 输送的样本中，激动剂诱导的血小板聚集显著升高（P<.02，所有激动剂）。RH 站点未发现显著差异（P>.05，所有激动剂）。对于高岭土 TEG，PTS 输送的样本显示 R 时间显著缩短和角度显著升高（P<.001）。MA 和 LY30 无显著差异（P>.05）。RapidTEG 的 ACT 显著降低（P=.001），功能性纤维蛋白原 TEG 的 MA 显著升高（P<.001）。肝素酶 TEG 检测无 PTS 显著影响（P>.05）。
根据 PTS 的类型，PTS 输送会影响 Multiplate 测量的血小板聚集。此外，PTS 改变了 TEG 参数，可能反映了凝血因子。临床实验室应评估当地 PTS 对 Multiplate 和 TEG 结果的影响。
