一种用于石蜡包埋组织电子显微镜检查的快速简便方法。
A rapid and simple method for electron microscopy of paraffin-embedded tissue.
作者信息
Widéhn S, Kindblom L G
机构信息
Department of Pathology, Sahlgren Hospital, University of Göteborg, Sweden.
出版信息
Ultrastruct Pathol. 1988 Jan-Feb;12(1):131-6. doi: 10.3109/01913128809048481.
A new rapid and simple method for electron microscopy of paraffin-embedded material is described. The paraffin-embedded tissue is deparaffinized in xylene, stained in a 0.01% toluidine blue/absolute ethanol solution, infiltrated in a propylene oxide/resin mixture, and embedded in epoxy resin. This quick method, which excludes rehydration, postosmification, and dehydration, takes about 3 h and produces results equal to previously described more laborious re-embedding techniques.
本文描述了一种用于石蜡包埋材料电子显微镜观察的快速简便新方法。将石蜡包埋组织在二甲苯中脱蜡，在0.01%甲苯胺蓝/无水乙醇溶液中染色，在环氧丙烷/树脂混合物中浸润，然后包埋在环氧树脂中。这种快速方法省去了复水、后固定和脱水步骤，耗时约3小时，所得结果与先前描述的更繁琐的重新包埋技术相当。
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