Departamento de Salud Pública, Universidad de Guadalajara, Camino Ramón Padilla Sánchez 2100, Zapopan, Jalisco, 45200, Mexico.
Departamento de Farmacobiología, Universidad de Guadalajara, Boulevard Marcelino García Barragán 1421, esq. Calzada Olímpica, Guadalajara, Jalisco, 44430, Mexico.
Environ Microbiol Rep. 2021 Aug;13(4):495-502. doi: 10.1111/1758-2229.12930. Epub 2021 Feb 4.
Salmonella Newport is a serovar frequently associated with outbreaks caused by consumption of raw tomatoes. This study tested the internalization of S. Newport-45 into cherry tomatoes and its resulting pathogenicity in vivo. Pathogenicity of S. Newport-45 was tested in BALB/c mice inoculated orally with either LB grown or cherry tomatoes homogenates internally contaminated with S. Newport-45. CFU of S. Newport-45 was recovered from the gastrointestinal tract, liver and spleen of the inoculated animals. Similar loads (p > 0.05) were recovered from the GI tract of BALB/c mice inoculated with S. Newport-45 grown in LB or with cherry tomato homogenates internally contaminated. Spread of S. Newport-45 to the liver of mice increased (p < 0.05) when they were inoculated with homogenates of cherry tomatoes internally contaminated with S. Newport-45 stored for 3 days compared with bacteria grown in LB. Salmonella Newport-45 hilA and rpoS genes were transcribed when the bacteria were inside the cherry tomato. The results obtained in this study show S. Newport-45 pathogenicity when it is internalized in a raw consumption fruit such as cherry tomato.
纽波特沙门氏菌是一种经常与食用生番茄引起的暴发有关的血清型。本研究测试了 S. Newport-45 进入樱桃番茄的内化及其在体内的致病性。通过口服接种 LB 生长或内部污染有 S. Newport-45 的樱桃番茄匀浆，在 BALB/c 小鼠中测试了 S. Newport-45 的致病性。从接种动物的胃肠道、肝脏和脾脏中回收了 S. Newport-45 的 CFU。从接种 LB 生长的 S. Newport-45 或内部污染的樱桃番茄匀浆的 BALB/c 小鼠胃肠道中回收的相同负荷（p>0.05）。当用内部污染有 S. Newport-45 的樱桃番茄匀浆接种时，S. Newport-45 向小鼠肝脏的传播增加（p<0.05），与在 LB 中生长的细菌相比。当细菌在樱桃番茄内时，S. Newport-45 hilA 和 rpoS 基因被转录。本研究获得的结果表明，当 S. Newport-45 在樱桃番茄等生食水果中内化时，它具有致病性。
