School of Public Health, University of South China, Hengyang, 421001, Hunan, China; Institute of Environment and Health, Shenzhen Center for Disease Control and Prevention, Shenzhen, 518055, Guangdong, China.
Institute of Environment and Health, Shenzhen Center for Disease Control and Prevention, Shenzhen, 518055, Guangdong, China; Xiangya School of Public Health, Central South University, Changsha, Hunan, 410078, China.
Environ Toxicol Pharmacol. 2021 May;84:103607. doi: 10.1016/j.etap.2021.103607. Epub 2021 Feb 2.
Human bronchial epithelial (HBE) cells and c-fos-silenced HBE cells were first exposed to fine particulate matter (PM) and the resulting miRNA sequenced. Thereafter, a weighted gene co-expression network analysis was performed using Cytoscape software to visualize the interactions between identified hub miRNAs and their target genes. Nine differentially expressed miRNAs in hub miRNAs were identified in the different treatment groups, of which miR-25-3p, miR-215-5p, and miR-145-5p were selected for further study. Following qPCR validation, both miR-25-3p and miR-215-5p were found to be significantly up-regulated whilst, miR-145-5p was significantly down-regulated (p < 0.05) in the PM group. Furthermore, miR-25-3p and miR-145-5p were also significantly down-regulated in the untreated group of c-fos silenced HBE cells. However, miR-215-5p was significantly down-regulated in both the untreated and PM-treated groups of c-fos silenced HBE cells. Subsequent analysis of their target genes also illustrated differential gene expression when comparing the treatment groups of the two cell types. The present data indicated that the c-fos gene has an important effect on the miRNA expression profiles and the related signaling pathways in PM-treated HBE cells. Therefore, each of miR-25-3p, miR-145-5p, and miR-215-5p may potentially provide future research information for additional exploration of a PM-induced carcinogenesis mechanism.
首先，将人支气管上皮（HBE）细胞和 c-fos 沉默的 HBE 细胞暴露于细颗粒物（PM）中，并对其产生的 miRNA 进行测序。然后，使用 Cytoscape 软件对鉴定的枢纽 miRNA 及其靶基因之间的相互作用进行加权基因共表达网络分析。在不同的处理组中，鉴定出 9 个差异表达的枢纽 miRNA 中的 miRNA-25-3p、miR-215-5p 和 miR-145-5p 进一步研究。经过 qPCR 验证，在 PM 组中发现 miR-25-3p 和 miR-215-5p 明显上调，而 miR-145-5p 明显下调（p<0.05）。此外，在未经处理的 c-fos 沉默的 HBE 细胞中，miR-25-3p 和 miR-145-5p 也明显下调。然而，在未经处理和 PM 处理的 c-fos 沉默的 HBE 细胞中，miR-215-5p 明显下调。随后对其靶基因的分析也表明，在两种细胞类型的处理组之间比较时，基因表达存在差异。本数据表明 c-fos 基因对 PM 处理的 HBE 细胞中的 miRNA 表达谱和相关信号通路有重要影响。因此，miR-25-3p、miR-145-5p 和 miR-215-5p 中的每一种都可能为进一步探索 PM 诱导的致癌机制提供未来的研究信息。
