Jilin Academy of Agricultural Sciences, China.
Jilin Academy of Agricultural Sciences, China.
Virology. 2021 Apr;556:33-38. doi: 10.1016/j.virol.2020.12.022. Epub 2021 Jan 28.
Five-week-old male BALB/c mice were inoculated intraperitoneally with a single (sP1) or multiple doses (mP1) of porcine circovirus-like virus P1 or cell culture medium. None of the mice exhibited clinical signs or gross lesions throughout the study. However, the body weights of the mP1 mice were significantly decreased, and the mice inoculated with P1 exhibited viral replication, seroconversion, and microscopic lesions. P1 nucleic acid was detected in the heart, liver, spleen, lung, bladder, testis, brain, thymus, and pancreatic tissues. Special P1 antibody was found in the P1-inoculated mice. Microscopic lesions in the sP1 and mP1 mice were characterized by interstitial pneumonia, including edema in the connective tissue around the pulmonary vessels, mild inflammatory cell infiltrate, thickened alveolar walls, myocardial necrosis, and dissolution of Purkinje cell nuclei. The results showed that the P1 virus could infect BALB/c mice. Thus, BALB/c mice may serve as models for P1 research.
五周龄雄性 BALB/c 小鼠经腹腔接种单次（sP1）或多次剂量（mP1）猪圆环病毒样病毒 P1 或细胞培养液。在整个研究过程中，没有小鼠出现临床症状或明显病变。然而，mP1 组小鼠的体重明显下降，接种 P1 的小鼠出现病毒复制、血清转化和显微镜下病变。P1 核酸在心脏、肝脏、脾脏、肺、膀胱、睾丸、大脑、胸腺和胰腺组织中均有检测到。在接种 P1 的小鼠中发现了特殊的 P1 抗体。sP1 和 mP1 组小鼠的显微镜下病变表现为间质性肺炎，包括肺血管周围结缔组织水肿、轻度炎性细胞浸润、肺泡壁增厚、心肌坏死和浦肯野细胞核溶解。结果表明，P1 病毒可以感染 BALB/c 小鼠。因此，BALB/c 小鼠可能成为 P1 研究的模型。
