Genetic Resource Centre, Latvian State Forest Research Institute "Silava", 111 Rigas st., LV-2169 Salaspils, Latvia.
Int J Mol Sci. 2021 Feb 3;22(4):1505. doi: 10.3390/ijms22041505.
Comparative transcriptome analysis provides a useful tool for the exploration of plant-pathogen interaction by allowing in-depth comparison of gene expression between unaffected, inoculated and wounded organisms. Here we present the results of comparative transcriptome analysis in genetically identical one-year-old Scots pine ramets after wounding and inoculation with . We identified 230 genes that were more than 2-fold upregulated in inoculated samples (compared to controls) and 116 downregulated genes. Comparison of inoculated samp les with wounded samples identified 32 differentially expressed genes (30 were upregulated after inoculation). Several of the genes upregulated after inoculation are involved in protection from oxidative stress, while genes involved in photosynthesis, water transport and drought stress tolerance were downregulated. An NRT3 family protein was the most upregulated transcript in response to both inoculation and wounding, while a U-box domain-containing protein gene was the most upregulated gene comparing inoculation to wounding. The observed transcriptome dynamics suggest involvement of auxin, ethylene, jasmonate, gibberellin and reactive oxygen species pathways and cell wall modification regulation in response to infection. The results are compared to methyl jasmonate induced transcriptome dynamics.
比较转录组分析通过在未受影响、接种和受伤的生物体之间深入比较基因表达，为研究植物-病原体相互作用提供了一种有用的工具。在这里，我们展示了在遗传上相同的一岁苏格兰松树无性系接种和受伤后进行比较转录组分析的结果。我们鉴定了 230 个在接种样本（与对照相比）中上调超过 2 倍的基因和 116 个下调基因。将接种样本与受伤样本进行比较，确定了 32 个差异表达基因（接种后有 30 个上调）。接种后上调的几个基因参与了抗氧化应激的保护，而光合作用、水分运输和耐旱性相关的基因则下调。NRT3 家族蛋白是对接种和受伤均反应上调最显著的转录本，而比较接种与受伤时，U -box 结构域蛋白基因是上调最显著的基因。观察到的转录组动态表明，在 感染后，生长素、乙烯、茉莉酸、赤霉素和活性氧途径以及细胞壁修饰调节参与其中。结果与茉莉酸甲酯诱导的转录组动态进行了比较。
