Bejar Ilan, Rubinstein Jacob, Bejar Jacob, Sabo Edmond, Sheffer Hilla, Bahouth Zaher, Halachmi Sarel, Vadasz Zahava
The Department of Urology, Shamir Medical Center, Be'er Ya'akov 6093000, Israel.
The Department of Mathematics Technion, Israeli Institute of Technology, Haifa 3200003, Israel.
Biology (Basel). 2021 Feb 3;10(2):109. doi: 10.3390/biology10020109.
Our previous studies showed elevated levels of Semaphorin3a (Sema3A) in the urine of patients with urothelial cancer compared to healthy patients. The aim of this study was to analyze the extent of Sema3A expression in normal and malignant urothelial tissue using immune-staining microscopic and morphometric analysis.
Fifty-seven paraffin-embedded bladder samples were retrieved from our pathology archive and analyzed: 14 samples of normal urothelium, 21 samples containing low-grade urothelial carcinoma, 13 samples of patients with high-grade urothelial carcinoma, 7 samples containing muscle invasive urothelial carcinoma, and 2 samples with pure urothelial carcinoma in situ. All samples were immunostained with anti Sema3A antibodies. The area of tissue stained with Sema3A and its intensity were analyzed using computerized morphometry and compared between the samples' groups.
In normal bladder tissue, very light Sema3A staining was demonstrated on the mucosal basal layer and completely disappeared on the apical layer. In low-grade tumor samples, cells in the basal layer of the mucosa were also lightly stained with Sema3A, but Seama3A expression intensified upon moving apically, reaching its highest level on apical cells exfoliating to the urine. In high grade urothelial tumors, Seama3A staining was intense in the entire thickness of the mucosa. In samples containing carcinoma in situ, staining intensity was high and homogenous in all the neoplastic cells.
Sema3A may be serve as a potential non-invasive marker of urothelial cancer.
我们之前的研究表明，与健康患者相比，尿路上皮癌患者尿液中的信号素3a（Sema3A）水平升高。本研究的目的是通过免疫染色显微镜和形态计量分析来分析正常和恶性尿路上皮组织中Sema3A的表达程度。
从我们的病理档案中检索出57个石蜡包埋的膀胱样本并进行分析：14个正常尿路上皮样本、21个包含低级别尿路上皮癌的样本、13个高级别尿路上皮癌患者的样本、7个包含肌层浸润性尿路上皮癌的样本以及2个纯原位尿路上皮癌样本。所有样本均用抗Sema3A抗体进行免疫染色。使用计算机形态计量学分析Sema3A染色的组织面积及其强度，并在样本组之间进行比较。
在正常膀胱组织中，Sema3A染色在黏膜基底层显示非常淡，在表层完全消失。在低级别肿瘤样本中，黏膜基底层的细胞也被Sema3A轻度染色，但Sema3A表达在向表层移动时增强，在脱落到尿液中的表层细胞上达到最高水平。在高级别尿路上皮肿瘤中，Sema3A染色在黏膜全层均强烈。在原位癌样本中，所有肿瘤细胞的染色强度高且均匀。
Sema3A可能作为尿路上皮癌的一种潜在非侵入性标志物。
