Reyes J, Benos D J
Departamento de Fisiologia y Biofisica, Facultad de Medicina, Universidad de Chile, Santiago.
Am J Physiol. 1988 Apr;254(4 Pt 1):C571-6. doi: 10.1152/ajpcell.1988.254.4.C571.
A comparative study of gossypol uncoupling of rat spermatogenic and liver cells shows that spermatogenic cells metabolizing pyruvate are two to three times more sensitive to gossypol uncoupling than either spermatogenic cells metabolizing glucose or liver cells metabolizing pyruvate or glucose. Direct measurements of in situ rat liver and spermatogenic cell mitochondrial respiration indicate that the pyruvate dependence of the gossypol uncoupling appears to be located in the spermatogenic cell mitochondria. A different type of mitochondrial uncoupler carbonyl cyanide m-chlorophenylhydrazone does not present the substrate-dependent uncoupling effect. This special interaction between spermatogenic cell pyruvate metabolism and gossypol uncoupling confers specificity to a bioenergetic model of gossypol action.
一项关于棉酚对大鼠生精细胞和肝细胞解偶联作用的比较研究表明，代谢丙酮酸的生精细胞对棉酚解偶联作用的敏感性比代谢葡萄糖的生精细胞或代谢丙酮酸或葡萄糖的肝细胞高两到三倍。对大鼠肝脏和生精细胞线粒体呼吸的原位直接测量表明，棉酚解偶联作用对丙酮酸的依赖性似乎位于生精细胞线粒体中。另一种类型的线粒体解偶联剂羰基氰化物间氯苯腙不存在底物依赖性解偶联效应。生精细胞丙酮酸代谢与棉酚解偶联之间的这种特殊相互作用赋予了棉酚作用生物能模型特异性。
