猪干扰素 delta 8 对体外伪狂犬病病毒的抗病毒活性。

Antiviral activity of porcine interferon delta 8 against pesudorabies virus in vitro.

机构信息

Henan Provincial Key Laboratory of Animal Immunology, Henan Academy of Agricultural Sciences, Zhengzhou, China; College of Veterinary Medicine, Northwest A&F University, Yangling, China.

Henan Provincial Key Laboratory of Animal Immunology, Henan Academy of Agricultural Sciences, Zhengzhou, China.

出版信息

Int J Biol Macromol. 2021 Apr 30;177:10-18. doi: 10.1016/j.ijbiomac.2021.01.208. Epub 2021 Feb 3.

DOI:10.1016/j.ijbiomac.2021.01.208

PMID:33548323

Abstract

Recently, pseudorabies virus (PRV) was isolated from human cases, and infected patients presented with respiratory dysfunction and acute neurological symptoms. However, there was no available effective drug to prevent the progression of PRV infection. In the present study, we screened a stably Drosophila S2 cell line which can secretory express a novel type I IFNs-interferon delta 8 (IFN-δ8) and the yield was about 10 mg/L. After purification, recombinant IFN-δ8 was demonstrated to be acid-stable, heat-stable, and nontoxic to PK-15 and 3D4/21 cells. Antiviral effects of IFN-δ8 against PRV were tested in vitro. Our results showed both pre- and post-treatment, recombinant PoIFN-δ8 exerted a significant protective effect against PRV infection in PK-15 and 3D4/21 cells. In addition, PoIFN-δ8 remarkably increased the expression of eight IFN-stimulated genes (ISGs), including ISG15, OAS1, PKR, MX1, CH25H, IFITM1, IFITM2 and IFITM3, to resist virus infection. These findings highlight the significance of IFN-δ8 that might serve as an antiviral agent for the prevention of PRV infection, and maybe expand the potential function of IFN antiviral drugs in the future.

摘要

最近，从人类病例中分离到了伪狂犬病毒（PRV），感染患者表现出呼吸功能障碍和急性神经症状。然而，目前还没有有效的药物可以预防 PRV 感染的进展。在本研究中，我们筛选了一个稳定的果蝇 S2 细胞系，该细胞系可以分泌表达一种新型 I 型干扰素-δ8（IFN-δ8），产量约为 10mg/L。经过纯化，重组 IFN-δ8 被证明具有耐酸、耐热和对 PK-15 和 3D4/21 细胞无毒的特性。我们在体外测试了 IFN-δ8 对 PRV 的抗病毒作用。结果表明，重组 PoIFN-δ8 无论是在预处理还是后处理时，对 PK-15 和 3D4/21 细胞中的 PRV 感染均具有显著的保护作用。此外，PoIFN-δ8 显著增加了 8 种干扰素刺激基因（ISGs）的表达，包括 ISG15、OAS1、PKR、MX1、CH25H、IFITM1、IFITM2 和 IFITM3，以抵抗病毒感染。这些发现强调了 IFN-δ8 的重要性，它可能作为预防 PRV 感染的抗病毒药物，并可能在未来扩展 IFN 抗病毒药物的潜在功能。