USERN Office, Abadan Faculty of Medical Sciences, Abadan, Iran.
Department of Parasitology, Faculty of Medical Sciences, Tarbiat Modares University, P. O. Box 14115-111, Tehran, Iran.
BMC Res Notes. 2021 Feb 6;14(1):50. doi: 10.1186/s13104-021-05467-1.
Toxoplasma gondii (T. gondii), an obligate intracellular apicomplexan parasite, could affect numerous warm-blooded animals, such as humans. Calcium-dependent protein kinases (CDPKs) are essential Ca signaling mediators and participate in parasite host cell egress, outer membrane motility, invasion, and cell division.
Several bioinformatics online servers were employed to analyze and predict the important properties of CDPK4 protein. The findings revealed that CDPK4 peptide has 1158 amino acid residues with average molecular weight (MW) of 126.331 KDa. The aliphatic index and GRAVY for this protein were estimated at 66.82 and - 0.650, respectively. The findings revealed that the CDPK4 protein comprised 30.14% and 34.97% alpha-helix, 59.84% and 53.54% random coils, and 10.02% and 11.49% extended strand with SOPMA and GOR4 tools, respectively. Ramachandran plot output showed 87.87%, 8.40%, and 3.73% of amino acid residues in the favored, allowed, and outlier regions, respectively. Also, several potential B and T-cell epitopes were predicted for CDPK4 protein through different bioinformatics tools. Also, antigenicity and allergenicity evaluation demonstrated that this protein has immunogenic and non-allergenic nature. This paper presents a basis for further studies, thereby provides a fundamental basis for the development of an effective vaccine against T. gondii infection.
刚地弓形虫（T. gondii）是一种专性细胞内顶复门寄生虫，可感染多种温血动物，包括人类。钙依赖蛋白激酶（CDPKs）是重要的钙信号转导介质，参与寄生虫宿主细胞逸出、外膜运动、入侵和细胞分裂。
使用了几种生物信息学在线服务器来分析和预测 CDPK4 蛋白的重要性质。研究结果表明，CDPK4 肽具有 1158 个氨基酸残基，平均分子量（MW）为 126.331 KDa。该蛋白的脂肪指数和 GRAVY 分别估计为 66.82 和-0.650。结果表明，CDPK4 蛋白由 30.14%和 34.97%的α-螺旋、59.84%和 53.54%的无规则卷曲和 10.02%和 11.49%的延伸链组成，分别使用 SOPMA 和 GOR4 工具。Ramachandran 图输出显示，氨基酸残基在优先、允许和异常区域的比例分别为 87.87%、8.40%和 3.73%。此外，还通过不同的生物信息学工具预测了 CDPK4 蛋白的多个潜在 B 和 T 细胞表位。同时，抗原性和变应原性评估表明，该蛋白具有免疫原性和非变应原性。本文为进一步的研究提供了基础，为开发有效的弓形虫感染疫苗提供了基础。
