Goldfinch M E, Maguire G A
Department of Clinical Biochemistry, Royal Victoria Infirmary, Newcastle upon Tyne, UK.
Ann Clin Biochem. 1988 Jan;25 ( Pt 1):73-7. doi: 10.1177/000456328802500110.
An investigation into the use of bilirubin oxidase to measure the apparent concentration of unbound bilirubin in solutions of bilirubin or bilirubin/human serum albumin demonstrated (1) near linearity of oxidation rate with bilirubin concentration up to 16 mumol/L, (2) linearity with respect to enzyme concentration up to 75 mg/L, (3) specificity of the enzyme for unbound bilirubin, as opposed to bound bilirubin, (4) poor precision. Very small absorbance changes leading to poor precision and the potential for interference by conjugated bilirubin meant that the method was unsuitable for patient samples.
一项关于使用胆红素氧化酶测量胆红素或胆红素/人血清白蛋白溶液中未结合胆红素表观浓度的研究表明：（1）胆红素浓度高达16 μmol/L时，氧化速率接近线性；（2）酶浓度高达75 mg/L时呈线性；（3）该酶对未结合胆红素具有特异性，与结合胆红素不同；（4）精密度较差。极小的吸光度变化导致精密度不佳，且结合胆红素可能产生干扰，这意味着该方法不适用于患者样本。
