Key Lab for Industrial Biocatalysis, Ministry of Education, Department of Chemical Engineering, Tsinghua University, Beijing 100084, China.
State Key Laboratory of Food Science and Technology, School of Food Science and Technology, Nanchang University, Nanchang 330047, China.
Trends Biotechnol. 2021 Nov;39(11):1173-1183. doi: 10.1016/j.tibtech.2021.01.002. Epub 2021 Feb 4.
Enzymatic catalysis, which has been driving biological processes in a green, mild, and efficient manner for billions of years, is increasingly being used in industrial processes to manufacture chemicals, pharmaceuticals, and materials for human society. Since enzymes were discovered, strategies to adapt enzymes for use as catalysts for industrial processes, such as chemical modification, immobilization, site-directed mutagenesis, directed evolution of enzymes, artificial metalloenzymes, and computational design, have been continuously pursued. In contrast to these strategies, editing enzymes to easily integrate biocatalysis with chemocatalysis is a potential way to apply enzymes in industry. Enzyme catalyst editing focuses on fine-tuning the microenvironment surrounding the enzyme or achieving a new catalytic function to construct better biocatalysis under non-natural conditions for the enzyme.
酶催化已经以绿色、温和、高效的方式驱动了数十亿年的生物过程，它正越来越多地被用于工业过程中，以制造化学品、药品和人类社会所需的材料。自发现酶以来，人们一直在不断探索将酶适应为工业过程催化剂的策略，例如化学修饰、固定化、定点突变、酶的定向进化、人工金属酶和计算设计。与这些策略相反，编辑酶以轻松地将生物催化与化学催化结合起来，是将酶应用于工业的一种潜在方法。酶催化剂编辑侧重于微调酶周围的微环境或实现新的催化功能，以在非自然条件下构建更好的生物催化。
