羊奶葡萄球菌产生肠毒素的定量研究。
A quantitative study of enterotoxin production by sheep milk staphylococci.
作者信息
Bautista L, Gaya P, Medina M, Nuñez M
机构信息
Departamento de Bioquímica y Microbiología, INIA, Madrid, Spain.
出版信息
Appl Environ Microbiol. 1988 Feb;54(2):566-9. doi: 10.1128/aem.54.2.566-569.1988.
Abstract
Of 124 staphylococcal strains isolated from sheep milk, 78 produced enterotoxin A, B, C, or D when evaluated by an enzyme-linked immunosorbent assay. Enterotoxins A and D, elaborated by 44 and 43 strains, respectively, showed the highest incidence. Enterotoxin production by coagulase-negative strains (one Staphylococcus cohnii, three S. epidermidis, five S. haemolyticus, and four S. xylosus) was detected. Linear and logarithmic-logarithmic regressions of optical density on enterotoxin concentration yielded the best-fitting equations for enterotoxin quantitation. A significantly higher incidence of enterotoxin producers and significantly higher levels of enterotoxins produced were recorded for coagulase-positive, thermostable nuclease-positive, hemolysis-positive, or mannitol-positive strains. Mannitol utilization was the best test for discriminating between enterotoxigenic and nonenterotoxigenic staphylococci.
摘要
在从羊奶中分离出的124株葡萄球菌菌株中，通过酶联免疫吸附测定法评估时，有78株产生了肠毒素A、B、C或D。分别由44株和43株产生的肠毒素A和D的发生率最高。检测到凝固酶阴性菌株（一株科氏葡萄球菌、三株表皮葡萄球菌、五株溶血葡萄球菌和四株木糖葡萄球菌）产生肠毒素。光密度对肠毒素浓度的线性和对数-对数回归得出了用于肠毒素定量的最佳拟合方程。凝固酶阳性、耐热核酸酶阳性、溶血阳性或甘露醇阳性菌株的肠毒素产生菌发生率显著更高，产生的肠毒素水平也显著更高。甘露醇利用是区分产肠毒素和不产肠毒素葡萄球菌的最佳试验。
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