Orden J A, Goyache J, Hernandez J, Domenech A, Suarez G, Gomez-Lucia E
Departamento de Patología Animal I Universidad Complutense, Madrid, Spain.
Zentralbl Veterinarmed B. 1992 Mar;39(2):144-8. doi: 10.1111/j.1439-0450.1992.tb01150.x.
The production of staphylococcal enterotoxins (SE) and toxic shock syndrome toxin-1 by 40 coagulase negative staphylococci isolated from sheep, goat and cow mastitis was studied. Both ELISA double sandwich and Western blot were used to detect the production of these toxins. Only two strains of S. xylosus were enterotoxigenic, producing SEC. TSST-1 was seen to be produced by 5 strains of S. xylosus, 1 S. sciuri and 2 S. epidermidis. Results obtained by ELISA and by Western blot agreed in all cases except in one strain of S. epidermidis which was only positive using ELISA.
对从绵羊、山羊和奶牛乳腺炎中分离出的40株凝固酶阴性葡萄球菌产生葡萄球菌肠毒素(SE)和中毒性休克综合征毒素-1的情况进行了研究。采用ELISA双夹心和蛋白质印迹法检测这些毒素的产生。仅两株木糖葡萄球菌产肠毒素,产生SEC。5株木糖葡萄球菌、1株松鼠葡萄球菌和2株表皮葡萄球菌可产生TSST-1。除1株表皮葡萄球菌仅ELISA检测呈阳性外,ELISA和蛋白质印迹法的检测结果在所有情况下均一致。