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使用来自免疫母鸡的蛋黄抗体通过酶联免疫吸附测定法对瘤胃细菌种类进行鉴别。

Differentiation of ruminal bacterial species by enzyme-linked immunosorbent assay using egg yolk antibodies from immunized chicken hens.

作者信息

Ricke S C, Schaefer D M, Cook M E, Kang K H

机构信息

Department of Meat and Animal Science, University of Wisconsin-Madison 53706.

出版信息

Appl Environ Microbiol. 1988 Feb;54(2):596-9. doi: 10.1128/aem.54.2.596-599.1988.

Abstract

Cross-reactivity among four species of ruminal bacteria was examined by using egg yolk antibodies from immunized Leghorn laying hens and an enzyme-linked-immunosorbent assay. The effects of the four species on the hens were compared on various days postimmunization. Hens injected with the same bacterial species had similar apparent antibody levels over the entire postimmunization period, but only Bacteroides ruminicola B1(4) and Selenomonas ruminantium D antigens elicited early increases in apparent antibody levels during weeks 2 and 3. Antibody cross-reactivity was greatly reduced by week 2, except for antibodies against Streptococcus bovis JB1.

摘要

利用来自免疫来亨蛋鸡的蛋黄抗体和酶联免疫吸附测定法,检测了四种瘤胃细菌之间的交叉反应性。在免疫后的不同天数,比较了这四种细菌对母鸡的影响。注射相同细菌种类的母鸡在整个免疫后期间具有相似的表观抗体水平,但只有瘤胃拟杆菌B1(4)和反刍月形单胞菌D抗原在第2周和第3周引起表观抗体水平的早期升高。到第2周时,抗体交叉反应性大大降低,除了抗牛链球菌JB1的抗体。

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本文引用的文献

4
VARIATION IN RUMEN BUTYRIVIBRIO STRAINS.瘤胃丁酸弧菌菌株的变异
J Bacteriol. 1964 Jun;87(6):1304-8. doi: 10.1128/jb.87.6.1304-1308.1964.
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Serological tests of a relationship between rumen selenomonads in vitro and in vivo.
J Gen Microbiol. 1962 Oct;29:265-70. doi: 10.1099/00221287-29-2-265.
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Maintenance of Laboratory strains of obligately anaerobic rumen bacteria.严格厌氧瘤胃细菌实验室菌株的保存
Appl Environ Microbiol. 1982 Aug;44(2):499-501. doi: 10.1128/aem.44.2.499-501.1982.

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