Polson A, von Wechmar M B, van Regenmortel M H
Immunol Commun. 1980;9(5):475-93. doi: 10.3109/08820138009066010.
Antibodies were isolated from the yolks of hens that were immunized with a variety of plant viruses by the use of polyethylene glycol (PEG). A concentration of 3.5% of the polymer caused the lipids and vitellin to separate, and the IgY was then precipitated with 12% PEG. The titre of the isolated antibody appears to remain at a high level after cessation of the course of immunization. Antibodies derived from the yolks of hens appear to have titres similar to those found in serum of rabbits immunized simultaneously. The observation made by several authors that a high salt concentration enhances fowl serum antibody precipitin titres could not be corroborated with 'yolk' antibodies directed against several plant viruses.
通过使用聚乙二醇（PEG）从用多种植物病毒免疫的母鸡的蛋黄中分离抗体。3.5%浓度的聚合物使脂质和卵黄磷蛋白分离，然后用12%的PEG沉淀IgY。在免疫过程停止后，分离出的抗体效价似乎保持在高水平。来自母鸡蛋黄的抗体效价似乎与同时免疫的兔子血清中的效价相似。几位作者观察到高盐浓度会提高禽血清抗体沉淀素效价，但针对几种植物病毒的“蛋黄”抗体并未证实这一点。
