Volkova Maria, Tsimafeyeu Ilya, Olshanskaya Anna, Khochenkova Yulia, Solomko Elyso, Ashuba Saida, Khochenkov Dmitry, Matveev Vsevolod
FSBI N.N. Blokhin National Medical Research Center for Oncology, Moscow, Russian Federation.
Institute of Oncology, Hadassah Medical Moscow, Moscow, Russian Federation.
Cent European J Urol. 2020;73(4):466-475. doi: 10.5173/ceju.0189.R1. Epub 2020 Dec 9.
The aim of our study was to investigate expression levels and the prognostic value of multiple growth factors and their receptors in the primary tumor cells of renal cell carcinoma (RCC).
Expression of vascular endothelial growth factor (VEGF)A, fibroblast growth factor (FGF)2, vascular endothelial growth factor receptor (VEGFR)1, VEGFR2, FGFR1, FGFR2, platelet-derived growth factor receptor (PDGFR)α, and PDGFRβ was investigated in 65 primary RCC specimens by immuhistochemical staining using the appropriate antibodies. Expression levels were evaluated by the semi-quantitative method. A search for correlations of expression levels of investigated growth factors and receptors with RCC features and patients outcomes was performed.
Expression of all growth factors and their receptors was detected both on the surface and in the cytoplasm of the primary tumor cells in RCC patients. The expression of all analyzed factors was interconnected. FGFR2 expression correlated with the largest number of other growth factors and receptors. A strong correlation was revealed between high expression of the studied markers, high Fuhrman grade, and advanced RCC stages. In a univariate analysis overexpression of VEGFR2 (p <0.0001) and FGFR2 (p = 0.014) had negative influence on cancer-specific survival.
Expression of growth factors and tyrosine kinase receptors in the primary tumor cells is strongly interconnected and associated with unfavorable features of RCC.
我们研究的目的是调查多种生长因子及其受体在肾细胞癌（RCC）原发肿瘤细胞中的表达水平及预后价值。
使用适当抗体通过免疫组织化学染色法，在65例RCC原发标本中研究血管内皮生长因子（VEGF）A、成纤维细胞生长因子（FGF）2、血管内皮生长因子受体（VEGFR）1、VEGFR2、FGFR1、FGFR2、血小板衍生生长因子受体（PDGFR）α和PDGFRβ的表达。采用半定量方法评估表达水平。对所研究的生长因子和受体的表达水平与RCC特征及患者预后的相关性进行了探究。
在RCC患者原发肿瘤细胞的表面和细胞质中均检测到所有生长因子及其受体的表达。所有分析因子的表达相互关联。FGFR2的表达与其他生长因子和受体的数量相关性最大。研究标记物的高表达、高Fuhrman分级和晚期RCC分期之间存在强相关性。在单因素分析中，VEGFR2（p <0.0001）和FGFR2（p = 0.014）的过表达对癌症特异性生存有负面影响。
原发肿瘤细胞中生长因子和酪氨酸激酶受体的表达紧密相连，且与RCC的不良特征相关。
