SREBP1 基因多态性对肉牛品种皮下脂肪组织脂肪酸组成及相关基因表达的影响。

Effect of a genetic polymorphism in SREBP1 on fatty acid composition and related gene expression in subcutaneous fat tissue of beef cattle breeds.

机构信息

Biomics Research Group, Lascaray Research Center, University of the Basque Country (UPV/EHU), Vitoria-Gasteiz, Spain.

Lactiker Research Group, Pharmacy & Food Sciences Department, University of the Basque Country (UPV/EHU), Vitoria-Gasteiz, Spain.

出版信息

Anim Sci J. 2021 Jan-Dec;92(1):e13521. doi: 10.1111/asj.13521.

DOI:10.1111/asj.13521

PMID:33554418

Abstract

Sterol regulatory element-binding factor 1 (SREBP1) plays an important role in the lipogenesis which affects fatty acid (FA) composition in backfat and consequently influences beef nutritional quality. This study analyzed the association of 84 bp-indel, both short (S) and long (L) alleles in intron 5 of SREBP1, with FA composition and gene expression of SREBP1 in backfat of northern Spanish beef breeds (Pirenaica, Salers and Holstein-Friesian). Phylogenetic analysis suggests that 84 bp-indel of ruminants is a highly conserved region compared with those in the full-length sequence of intron 5 or mRNA of SREBP1 among species. Overall, higher content of polyunsaturated FAs was observed in SL genotype compared to LL genotype of 84 bp-Indel (p < .05). In particular, in Pirenaica, SL genotype was associated with a higher content of stearic (18:0), α-linolenic (18:3n-3) acid, and total n-3 content (p < .05). However, the gene expression of SREBP1 did not differ among genotypes of 84 bp-Indel (p > .05).

摘要

固醇调节元件结合蛋白 1（SREBP1）在脂肪生成中起着重要作用，影响背膘中的脂肪酸（FA）组成，从而影响牛肉的营养品质。本研究分析了西班牙北部肉牛品种（比利牛斯牛、萨莱尔牛和荷斯坦-弗里生牛）背膘中 SREBP1 第 5 内含子 84bp 插入/缺失（84bp-indel），包括短（S）和长（L）等位基因，与 FA 组成和 SREBP1 基因表达的关系。系统发育分析表明，与物种间 SREBP1 第 5 内含子全长序列或 mRNA 的 84bp 插入/缺失相比，84bp 插入/缺失在反刍动物中是一个高度保守的区域。总体而言，84bp-Indel 的 SL 基因型与 LL 基因型相比，多不饱和 FA 的含量更高（p<0.05）。特别是在比利牛斯牛中，SL 基因型与硬脂酸（18:0）、α-亚麻酸（18:3n-3）和总 n-3 含量的增加有关（p<0.05）。然而，84bp-Indel 各基因型之间 SREBP1 的基因表达没有差异（p>0.05）。