小牛子宫雌激素受体非甾体结合成分的序列分析

Sequence analysis of the nonsteroid binding component of the calf uterine estrogen receptor.

作者信息

Ratajczak T, Brockway M J, Hähnel R, Moritz R L, Simpson R J

机构信息

Department of Obstetrics and Gynaecology, University of Western Australia, King Edward Memorial Hospital for Women, Subiaco.

出版信息

Biochem Biophys Res Commun. 1988 Mar 30;151(3):1156-63. doi: 10.1016/s0006-291x(88)80487-x.

DOI:10.1016/s0006-291x(88)80487-x

PMID:3355547

Abstract

Microbore reversed-phase high performance liquid chromatography has been utilized to fractionate and purify a number of tryptic peptides generated from the 90K nonsteroid binding component of the calf uterine estrogen receptor. Sequence analysis was performed on six peptides yielding 78 unique amino acid assignments, this corresponds to approximately 10% of the molecule. These peptides share sequence similarities with three heat shock proteins, Drosophila hsp 83 (83% homologous), yeast hsp 90 (55%) and chicken hsp 108 (32%). The amino acid composition of the protein indicates a prevalence of charged amino acid residues.

摘要

微径反相高效液相色谱法已被用于分离和纯化从小牛子宫雌激素受体的90K非类固醇结合成分产生的多种胰蛋白酶肽。对六种肽进行了序列分析，得到了78个独特的氨基酸归属，这大约相当于该分子的10%。这些肽与三种热休克蛋白具有序列相似性，果蝇热休克蛋白83（同源性83%）、酵母热休克蛋白90（55%）和鸡热休克蛋白108（32%）。该蛋白质的氨基酸组成表明带电荷的氨基酸残基占优势。