Dutton Haley R, Du Preez Louis H, Verneau Olivier, Whelan Nathan V, Bullard Stephen A
Auburn University, Aquatic Parasitology Laboratory and Southeastern Cooperative Fish Parasite and Disease Laboratory, School of Fisheries, Aquaculture, & Aquatic Sciences, College of Agriculture, Auburn University, 203 Swingle Hall, Auburn, Alabama 36849.
African Amphibian Conservation Research Group, Unit for Environmental Sciences and Management, North-West University, Private Bag X6001, Potchefstroom, 2520, South Africa.
J Parasitol. 2021 Jan 1;107(1):74-88. doi: 10.1645/20-96.
Herein, we describe several newly-collected specimens of Neopolystoma cf. orbiculare from the urinary bladder of 2 alligator snapping turtles, Macrochelys temminckii (Troost in Harland, 1835) (Cryptodira: Chelydridae Gray, 1831) from Comet Lake (30°35'46.94″N, 88°36'3.12″W), Pascagoula River, Mississippi. Our specimens differed from all previous descriptions of N. orbiculare and its junior subjective synonyms by the combination of having intestinal ceca adorned with triangular pockets and that terminate dorsal to the haptor, distinctive hooklets each having a handle and guard of approximately equal length and having a much longer and curved blade, 16 genital coronet spines that each possess 1-2 flanges per spine, pre-testicular vaginal pores, and vaginal ducts that are anterior to the junction of the oviduct and genito-intestinal canal. Some of our specimens were enantiomorphic (4 and 3 had a dextral and sinistral ovary, respectively). Nucleotide sequences (large subunit ribosomal DNA [28S], small subunit ribosomal DNA [18S], and cytochrome oxidase subunit 1 mitochondrial gene [COI]) for our specimens were most similar to GenBank sequences ascribed to N. orbiculare. Single-gene and concatenated phylogenetic analyses confirmed that NeopolystomaPrice, 1939 is polyphyletic and that our isolates share a recent common ancestor with those ascribed to N. orbiculare. This is the first record of a polystomatid from Mississippi, from the Pascagoula River, and from the alligator snapping turtle (and only the second species of Neopolystoma reported from any snapping turtle).
在此，我们描述了从密西西比州帕斯卡古拉河彗星湖（北纬30°35'46.94″，西经88°36'3.12″）的两只大鳄龟（Macrochelys temminckii (Troost in Harland, 1835)，龟鳖目：鳄龟科Gray, 1831）膀胱中新采集的几例近似圆形新多盘吸虫（Neopolystoma cf. orbiculare）标本。我们的标本与之前所有关于圆形新多盘吸虫及其次主观同物异名的描述不同，其特征组合包括：肠盲囊饰有三角形袋并终止于吸附器背侧；独特的小钩，每个小钩的柄和护片长度大致相等，且刀片更长且弯曲；16根生殖冠状棘，每根棘有1 - 2个凸缘；睾丸前阴道孔；以及位于输卵管与生殖肠管交界处前方的阴道管。我们的一些标本是镜像对称的（分别有4个和3个标本的卵巢为右旋和左旋）。我们标本的核苷酸序列（大亚基核糖体DNA [28S]、小亚基核糖体DNA [18S]和细胞色素氧化酶亚基1线粒体基因[COI]）与GenBank中归为圆形新多盘吸虫的序列最为相似。单基因和串联系统发育分析证实，1939年的新多盘吸虫属（Neopolystoma Price）是多系的，并且我们分离出的菌株与归为圆形新多盘吸虫的菌株有最近的共同祖先。这是来自密西西比州、帕斯卡古拉河以及大鳄龟的多盘吸虫的首次记录（也是从任何鳄龟中报道的新多盘吸虫属的第二个物种）。
