利用小米糠和菜籽粕水解物生产聚（3-羟基丁酸酯）的巨大芽孢杆菌的重组生产。
Recombinant production of poly-(3-hydroxybutyrate) by Bacillus megaterium utilizing millet bran and rapeseed meal hydrolysates.
机构信息
BioPAT Laboratory, Department of Biosciences and Bioengineering, Indian Institute of Technology Guwahati, Guwahati 781039, Assam, India.
Department of Biotechnology, Manipal Institute of Technology, Manipal 576104, Karnataka, India.
出版信息
Bioresour Technol. 2021 Apr;326:124800. doi: 10.1016/j.biortech.2021.124800. Epub 2021 Feb 2.
Fermentative poly-3-hydroxybutyrate (PHB) production is mainly limited by the cost of raw material. In this present study, low-cost feedstock viz., millet bran residue (MBRH) and rapeseed meal hydrolysates were successfully utilized for PHB production. Metabolic engineering of Bacillus megaterium by co-expression of both precursor (phbRBC) and NADPH cofactor regeneration (zwf) genes resulted in 2.67-fold enhancement in PHB accumulation compared to wild strain. Modified logistic model characterized B.megaterium growth and PHB production effectively. The kinetic analysis proved that maximum cell concentration (15.01 g.L) and growth-associated constant (0.22 g.g) were found to be higher for initial MBRH concentration (S = 20 g.L). PHB production kinetics elucidated its expression in B.megaterium was growth-associated. PHB synthesized by B.megaterium was characterized using FTIR, NMR, XRD, DSC/TGA, FESEM and the physio-chemical properties enumerated its as a potential biodegradable plastic for industrial application.
发酵聚 3-羟基丁酸酯（PHB）的生产主要受到原材料成本的限制。在本研究中，成功利用了低成本的原料，即小米糠渣（MBRH）和菜籽粕水解物，用于 PHB 的生产。通过共表达前体（phbRBC）和 NADPH 辅助因子再生（zwf）基因对巨大芽孢杆菌进行代谢工程改造，与野生菌株相比，PHB 的积累提高了 2.67 倍。改良的 logistic 模型有效地描述了 B.megaterium 的生长和 PHB 生产过程。动力学分析表明，对于初始 MBRH 浓度（S=20g/L），最大细胞浓度（15.01 g/L）和与生长相关的常数（0.22 g/g）更高。PHB 在 B.megaterium 中的表达被证明与生长相关。使用 FTIR、NMR、XRD、DSC/TGA、FESEM 对 B.megaterium 合成的 PHB 进行了表征，列举了其理化性质，说明它是一种具有工业应用潜力的可生物降解塑料。
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