Department of Health Sciences, Università del Piemonte Orientale, Novara, Italy.
Department of Health Sciences, Università del Piemonte Orientale, Novara, Italy.
Surg Oncol. 2021 Jun;37:101524. doi: 10.1016/j.suronc.2021.101524. Epub 2021 Jan 31.
Colorectal cancer is still one of the leading causes of cancer-related deaths worldwide and specific tools to improve disease assessment and treatment response are advocated. The aim of this study was to analyze miRNAs expression in metastatic and non-metastatic colorectal cancer, in order to identify reliable biomarkers suitable for prognosis, treatment and patient's monitoring.
Among 25 patients (mean age 71 ± 12 years; Female/Male: 12/13) submitted to laparoscopic colorectal resection between August 2017 and February 2019, miRNAs were extracted from fresh frozen tissues of solid tumors. Gene expression's analysis arising from microarray technology was performed.
Pathological evaluation identified 15 metastatic patients (8 presenting with stage III and 7 with stage IV cancers) and 10 non-metastatic patients (4 presenting with stage I and 6 stage II cancers). Bioinformatic analysis of solid biopsies revealed 16 miRNAs (11 upregulated and 5 downregulated) differently expressed between metastatic and non-metastatic groups, with three miRNAs (miR-7515, miR-7109-5p and miR-6831-5p) never linked to colorectal cancer before.
Our study showed an association between miRNAs and metastatic colorectal cancer, suggesting their potential role as biomarkers for tumor management, if confirmed by further studies.
结直肠癌仍然是全球癌症相关死亡的主要原因之一，因此提倡使用特定的工具来改善疾病评估和治疗反应。本研究旨在分析转移性和非转移性结直肠癌中的 miRNA 表达，以确定适合预后、治疗和患者监测的可靠生物标志物。
在 2017 年 8 月至 2019 年 2 月期间接受腹腔镜结直肠切除术的 25 名患者（平均年龄 71 ± 12 岁；女性/男性：12/13）中，从实体肿瘤的新鲜冷冻组织中提取 miRNA。采用微阵列技术进行基因表达分析。
病理评估确定了 15 名转移性患者（8 名 III 期和 7 名 IV 期癌症患者）和 10 名非转移性患者（4 名 I 期和 6 名 II 期癌症患者）。对实体活检的生物信息学分析显示，转移性和非转移性组之间有 16 个 miRNA（11 个上调和 5 个下调）表达不同，其中有 3 个 miRNA（miR-7515、miR-7109-5p 和 miR-6831-5p）以前从未与结直肠癌相关联。
我们的研究表明 miRNA 与转移性结直肠癌之间存在关联，提示它们具有作为肿瘤管理生物标志物的潜力，如果进一步的研究得到证实。
