大鼠肝脏中溶酶体糖基天冬酰胺酶的周转
The turnover of lysosomal glycosylasparaginase in rat liver.
作者信息
Tollersrud O K, Hofmann S H, Aronson N N
机构信息
Department of Molecular and Cell Biology, Pennsylvania State University, University Park.
出版信息
Biochim Biophys Acta. 1988 Apr 14;953(3):353-6. doi: 10.1016/0167-4838(88)90044-1.
Rat liver glycosylasparaginase (N4-(beta-N-acetyl-D-glucosaminyl)-L-asparagine amidohydrolase (EC 3.5.1.26) was irreversibly inhibited in vitro by the asparagine analogue 5-diazo-4-oxo-L-norvaline (DONV). Following the abolition of this lysosomal hydrolase by DONV in rats, the enzyme activity in liver recovered exponentially. Assuming a zero order rate of protein synthesis, the half-life of the glycosylasparaginase in rat liver was measured to be 2 days.
大鼠肝脏糖基天冬酰胺酶（N4-（β-N-乙酰-D-葡糖胺基）-L-天冬酰胺酰胺水解酶，EC 3.5.1.26）在体外被天冬酰胺类似物5-重氮-4-氧代-L-正缬氨酸（DONV）不可逆地抑制。DONV使大鼠体内这种溶酶体水解酶失活后，肝脏中的酶活性呈指数恢复。假设蛋白质合成速率为零级，测得大鼠肝脏中糖基天冬酰胺酶的半衰期为2天。
Zotero 插件