Ness G C, Pendleton L C, McCreery M J
Department of Biochemistry, College of Medicine, University of South Florida, Tampa 33612.
Biochim Biophys Acta. 1988 Apr 14;953(3):361-4. doi: 10.1016/0167-4838(88)90046-5.
Radiation inactivation analysis of liver pieces yielded a target size of 210 kDa for hepatic 3-hydroxy-3-methylglutaryl CoA (HMG-CoA) reductase [S)-mevalonate:NADP+ oxidoreductase (CoA-acylating), EC 1.1.1.34) from rats fed a normal diet. Feeding a diet containing mevinolin and colestipol, which causes a marked increase in enzyme activity, resulted in a reduction of the target size to 120 kDa. These results are consistent with those obtained by radiation inactivation and immunoblotting analysis of isolated microsomes and suggest that the increase in HMG-CoA reductase activity caused by these dietary agents is accompanied by a change from a dimer to a monomer form of the enzyme.
对肝组织块进行辐射失活分析得出，正常饮食喂养的大鼠肝脏中3-羟基-3-甲基戊二酰辅酶A（HMG-CoA）还原酶[S)-甲羟戊酸:NADP+氧化还原酶（辅酶A酰化），EC 1.1.1.34]的靶标大小为210 kDa。喂食含有美伐他汀和考来替泊的饮食，这会导致酶活性显著增加，结果靶标大小降至120 kDa。这些结果与对分离的微粒体进行辐射失活和免疫印迹分析所得到的结果一致，并表明这些饮食因素引起的HMG-CoA还原酶活性增加伴随着酶从二聚体形式转变为单体形式。
