一种新型含恶唑的四烯化合物 JBIR-159，由隐藏的反式 AT 型聚酮合酶生物合成基因簇的异源表达产生。
A novel oxazole-containing tetraene compound, JBIR-159, produced by heterologous expression of the cryptic trans-AT type polyketide synthase biosynthetic gene cluster.
机构信息
National Institute of Advanced Industrial Science and Technology, Tokyo, Japan.
Japan Biological Informatics Consortium (JBIC), Tokyo, Japan.
出版信息
J Antibiot (Tokyo). 2021 May;74(5):354-358. doi: 10.1038/s41429-021-00410-9. Epub 2021 Feb 8.
Using genome mining approach, we identified a novel biosynthetic gene cluster containing trans-AT type PKS genes from Streptomyces versipellis 4083-SVS6. A bacterial artificial chromosome (BAC) clone, pKU503JL68_PN1_P10-C12, accommodating the entire biosynthetic gene cluster was obtained from a BAC library. Heterologous expression of the biosynthetic gene cluster in Streptomyces lividans TK23 led to the production of a novel polyene compound, JBIR-159. We report herein the biosynthetic gene cluster for JBIR-159, and the heterologous expression, isolation, structure determination and a brief biological activity.
利用基因组挖掘方法，我们从链霉菌（Streptomyces versipellis 4083-SVS6）中鉴定出一个新型生物合成基因簇，其中包含跨 AT 型 PKS 基因。从 BAC 文库中获得了一个容纳整个生物合成基因簇的细菌人工染色体（BAC）克隆，pKU503JL68_PN1_P10-C12。在链霉菌（Streptomyces lividans TK23）中异源表达生物合成基因簇导致了一种新型多烯化合物 JBIR-159 的产生。本文报道了 JBIR-159 的生物合成基因簇，以及异源表达、分离、结构确定和简要的生物活性。
Zotero 插件