State Key Laboratory of Pharmaceutical Biotechnology, Institute of Functional Biomolecules, School of Life Sciences , Nanjing University , Nanjing 210023 , China.
State Key Laboratory Cultivation Base for TCM Quality and Efficacy , Nanjing University of Chinese Medicine , Nanjing 210023 , China.
Org Lett. 2019 May 17;21(10):3785-3788. doi: 10.1021/acs.orglett.9b01237. Epub 2019 Apr 29.
Genome mining of the marine Streptomyces seoulensis A01 enabled the identification of a giant type I polyketide synthase gene cluster ( asm). Heterologous expression of the cryptic asm cluster using a bacterial artificial chromosome vector in heterologous host led to the production of ansaseomycins A (1) and B (2). A plausible biosynthetic pathway was also proposed. Additionally, compounds 1 and 2 are active against K562 cell lines with IC values of 13.3 and 18.1 μM, respectively.
海洋链霉菌 A01 的基因组挖掘使得一个巨型 I 型聚酮合酶基因簇（asm）被鉴定出来。利用细菌人工染色体载体在异源宿主中异源表达这个隐藏的 asm 簇，导致了 ansaseomycins A（1）和 B（2）的产生。还提出了一个合理的生物合成途径。此外，化合物 1 和 2 对 K562 细胞系具有活性，IC 值分别为 13.3 和 18.1 μM。
