College of Pharmacy, Nanjing University of Chinese Medicine, 138 Xianlin Road, Nanjing 210023, P.R. China.
J Chromatogr Sci. 2021 Sep 29;59(9):847-855. doi: 10.1093/chromsci/bmab006.
Fructus Gardeniae, known as Zhi-zi in China, has been used as Chinese herbal medicine and functional health food for thousands of years. Fructus Gardeniae Grandiflorae, named as Shui-zhizi, is a counterfeit herb of Fructus Gardeniae. In order to discriminate these two varieties, based on ultra-high-performance liquid chromatography, an analysis method of fingerprints of Fructus Gardeniae and Fructus Gardeniae Grandiflorae was established. With hierarchical clustering analysis and principal component analysis, they were separated into two groups. Analyzed with partial least squares discriminant analysis, there were differences in chemical compositions between Fructus Gardeniae and Fructus Gardeniae Grandiflorae. Six compounds, crocin I, genipin-1-β-D-gentiobioside and four other unknown compositions were identified as differential marker compositions between them. Furthermore, seven active substances in them were determined simultaneously. Thus, an integral method of ultra-high-performance liquid chromatography fingerprint combined with chemometrics analysis and quantitative assessment was established. It could be utilized in characterization, quality evaluation of Fructus Gardeniae and could be applied for discriminating Fructus Gardeniae from Fructus Gardeniae Grandiflorae.
栀子，在中国又被称为“栀子”，作为中草药和功能性保健食品已经有几千年的历史了。栀子的一种伪品是大花栀子，又名水栀子。为了鉴别这两种品种，本研究基于超高效液相色谱法，建立了栀子及其伪品大花栀子的指纹图谱分析方法。通过层次聚类分析和主成分分析，它们被分为两组。通过偏最小二乘判别分析，栀子和大花栀子在化学成分上存在差异。鉴定出 6 种化合物，包括藏红花酸 I、京尼平-1-β-D-龙胆二糖苷和另外 4 种未知成分，这些都是它们之间的差异标记成分。此外，还同时测定了它们中的 7 种活性物质。因此，建立了一种超高效液相色谱指纹图谱结合化学计量学分析和定量评价的整体方法。该方法可用于栀子的特征描述和质量评价，也可用于鉴别栀子及其伪品大花栀子。
