Department of Biochemistry, University of Allahabad, Allahabad, Uttar Pradesh, India.
CSIR-Central Salt & Marine Chemicals Research Institute, Bhavnagar, Gujarat, India.
J Basic Clin Physiol Pharmacol. 2021 Feb 8;33(2):163-167. doi: 10.1515/jbcpp-2020-0100.
Piperine (1-piperoyl piperidine), a major alkaloid constituent of L. and L. has pleiotropic biological effects, but the mechanism(s) involved remain to be elucidated. The current study was conducted to examine the efficacy of antioxidant ability of piperine on t-BHP induced markers of oxidative stress in human erythrocytes.
Healthy human erythrocytes and erythrocytes membrane was stressed with free radical inducer chemical; BHP (10 M), and the effects of piperine was measured against free radical mediated modification in lipid and protein content, -SH and GSH value with antioxidant potential.
The results demonstrate that treatment of erythrocytes with piperine (10 to 10 M) significantly (p<0.05) ameliorated the adverse consequences of oxidative stress as evidenced by prevention of oxidation of erythrocyte reduced glutathione, membrane thiols, proteins, and peroxidation of lipids; the effects were in correlation with ferric reducing and radical scavenging abilities of piperine.
The study concludes that piperine possesses potent anti-oxidant potential which may explain many of its observed biological effects.
胡椒碱（1-哌啶酰基哌啶）是 L. 和 L. 的主要生物碱成分，具有多种生物学效应，但相关机制仍有待阐明。本研究旨在探讨胡椒碱抗氧化能力对 t-BHP 诱导的人红细胞氧化应激标志物的疗效。
用自由基诱导剂化学物质 BHP（10μM）对健康人红细胞和红细胞膜进行应激处理，并测量胡椒碱对脂质和蛋白质含量、-SH 和 GSH 值的自由基介导修饰以及抗氧化能力的影响。
结果表明，胡椒碱（10 至 10μM）处理红细胞可显著（p<0.05）减轻氧化应激的不利后果，如红细胞还原型谷胱甘肽、膜巯基、蛋白质和脂质过氧化的氧化作用得到预防；这些作用与胡椒碱的铁还原和自由基清除能力相关。
研究结论表明，胡椒碱具有强大的抗氧化潜力，这可以解释其许多观察到的生物学效应。
