Department of Information Technology, JIS College of Engineering, Kalyani, West Bengal, India.
Department of Biochemistry and Biophysics, University of Kalyani, Kalyani, West Bengal, India.
Appl Biochem Biotechnol. 2021 Jul;193(7):2076-2086. doi: 10.1007/s12010-021-03520-0. Epub 2021 Feb 9.
Circular RNAs belong to the class of non-coding RNA molecules, though surprisingly some of them have protein-coding potentials. However, the circular RNA circ-SHPRH is known to code for an unusual protein known as SHPRH-146aa. However, the molecular level details of the protein are not yet identified. It was proposed that the protein has its role in glioblastoma. Therefore, in this work, an attempt was made to decipher the various structural features of SHPRH-146aa. The binding interactions of the protein SHPRH-146aa with its partner protein DTL were also analyzed. The main aim of the work was to decipher the characteristics features of this unusual protein and the region on SHPRH-146aa that would form different types of non-covalent binding interactions both among itself as well as with its binding partner. In this work, we tried to elucidate the various structural and physico-chemical features of the protein as well as its mode of interactions with its binding partner. The study would therefore pave the pathway to design future wet lab experiments to delineate the appropriate structural features of the protein as well as its association with glioblastoma and neuro-degenerative diseases.
环状 RNA 属于非编码 RNA 分子一类，尽管令人惊讶的是，其中一些具有蛋白质编码潜力。然而，环状 RNA circ-SHPRH 已知编码一种称为 SHPRH-146aa 的不寻常蛋白质。然而，该蛋白质的分子水平细节尚不清楚。有人提出该蛋白在神经胶质瘤中起作用。因此，在这项工作中，试图破译 SHPRH-146aa 的各种结构特征。还分析了蛋白质 SHPRH-146aa 与其伴侣蛋白 DTL 之间的结合相互作用。这项工作的主要目的是破译这种不寻常蛋白质的特征，并确定 SHPRH-146aa 上的区域，该区域将形成不同类型的非共价结合相互作用，包括其自身以及与其结合伴侣之间的相互作用。在这项工作中，我们试图阐明蛋白质的各种结构和物理化学特性及其与结合伴侣相互作用的模式。因此，该研究将为设计未来的湿实验室实验铺平道路，以描绘蛋白质的适当结构特征及其与神经胶质瘤和神经退行性疾病的关联。
