Department of Oral Biochemistry, Academic Centre for Dentistry Amsterdam, Free University and University of Amsterdam, G. Mahlerlaan 3004, 1081LA Amsterdam, the Netherlands.
Biochem Cell Biol. 2021 Feb;99(1):128-137. doi: 10.1139/bcb-2020-0285. Epub 2021 Feb 9.
Saliva is essential for the maintenance of oral health. When salivary flow is impaired, the risk of various oral diseases such as caries and candidiasis increases drastically. Under healthy conditions, saliva provides effective protection against microbial colonization by the collaborative action of numerous host-defense molecules. This review describes how saliva has been the guideline for the design and characterization of a heterodimeric antimicrobial construct called LFchimera. This construct mimics the helical parts of two antimicrobial domains in the crystal structure of bovine lactoferrin. It shows high antimicrobial activity against a broad spectrum of Gram-positive and Gram-negative bacteria, fungi, and parasites including biowarfare agents such as , , and . Further, sublethal concentrations of LFchimera inhibited biofilm formation, the invasiveness of HeLa cells by spp., and prevented haemolysis of enteropathogenic , demonstrating the versatility of these peptides.
唾液对于维护口腔健康至关重要。当唾液流量受损时，龋齿和念珠菌病等各种口腔疾病的风险会大幅增加。在健康状况下，唾液通过多种宿主防御分子的协同作用，为微生物定植提供了有效的保护。本综述描述了唾液如何成为设计和表征一种称为 LFchimera 的异二聚体抗菌构建体的指南。该构建体模拟了牛乳铁蛋白晶体结构中两个抗菌结构域的螺旋部分。它对广谱革兰氏阳性和革兰氏阴性细菌、真菌和寄生虫具有高抗菌活性，包括生物战剂如炭疽、天花和肉毒梭菌。此外，LFchimera 的亚致死浓度抑制了生物膜的形成、 spp.对 HeLa 细胞的侵袭性，并防止了肠致病性 的溶血，证明了这些肽的多功能性。
