左旋肉碱对喂食高胆固醇饮食大鼠载脂蛋白模式的影响。

The effect of L-carnitine on the apolipoprotein pattern of rats fed a cholesterol-rich diet.

Mondola P, Belfiore A, Santangelo F, Santillo M

Institute of Physiology, Medical Faculty, Naples University, Italy.

Comp Biochem Physiol B. 1988;89(1):69-73. doi: 10.1016/0305-0491(88)90263-5.

Abstract

L-Carnitine was administered to hypercholesterolemic rats for eight days, after which the blood was collected by intracardiac puncture and lipoproteins were collected by preparative ultracentrifuge. 2. The chemical composition of lipoproteins of normal rats and hypercholesterolemic rats before and after L-carnitine treatment was determined. 3. The total serum B and E apoproteins were assayed by rocket electrophoresis, while apoproteins in VLDL, IDL, LDL and HDL were determined by densitometric scanning of SDS and TMU polyacrylamide disc gel electrophoresis. 4. The results obtained showed that the L-carnitine treatment tends to restore to normal values both the chemical composition of lipoproteins and the apoproteins pattern of rats fed on a diet enriched with cholesterol.

摘要

给高胆固醇血症大鼠施用左旋肉碱八天，之后通过心脏穿刺采集血液，并通过制备型超速离心机收集脂蛋白。2. 测定了正常大鼠和高胆固醇血症大鼠在左旋肉碱治疗前后脂蛋白的化学组成。3. 通过火箭电泳测定血清总B和E载脂蛋白，而通过SDS和TMU聚丙烯酰胺圆盘凝胶电泳的光密度扫描测定极低密度脂蛋白（VLDL）、中间密度脂蛋白（IDL）、低密度脂蛋白（LDL）和高密度脂蛋白（HDL）中的载脂蛋白。4. 所得结果表明，左旋肉碱治疗倾向于使喂食富含胆固醇饮食的大鼠的脂蛋白化学组成和载脂蛋白模式恢复到正常值。