用胰蛋白酶对年轻人和老年人红细胞释放的主要膜表面唾液酸糖肽进行的比较研究。

Comparative study on the main membrane-surface sialoglycopeptides released from young and old human erythrocytes with trypsin.

Shinozuka T, Takei S, Yanagida J, Watanabe H, Ohkuma S

Department of Legal Medicine, School of Medicine, Keio University, Japan.

Comp Biochem Physiol B. 1988;89(2):309-15. doi: 10.1016/0305-0491(88)90228-3.

Abstract

The amount of sialoglycopeptide (SGP) mixture released from young (Y) erythrocytes by trypsin treatment was significantly larger than that from old (O) ones. 2. The two main membrane-surface sialoglycopeptides (Y-SGP and O-SGP) were isolated in yields of about 45% and 42% from the mixtures by a combination of Sephadex G-50 superfine gel filtration with DEAE-cellulose chromatography, respectively. Molecular weights of 12,000 and 12,100, respectively, were found for the Y-SGP and the O-SGP. 3. The Y-SGP showed a greater proportion of sialic acid than the O-SGP. The contents of galactose, N-acetylglucosamine and N-acetylgalactosamine of the Y-SGP were significantly lower than those of the O-SGP. 4. Both the SGPs exhibited the same reactivities against the MN reagents, but the reactivities against the lectins of Limulus polyphemus and Phaseolus vulgaris (E) were stronger in the Y-SGP than in the O-SGP. Neither the Y-SGP nor the O-SGP showed the reactivity to Arachis hypogaea (anti-T) lectin.

摘要

经胰蛋白酶处理后，年轻（Y）红细胞释放的唾液糖肽（SGP）混合物的量显著大于年老（O）红细胞释放的量。2. 通过葡聚糖凝胶G - 50超细凝胶过滤与二乙氨基乙基纤维素色谱法相结合，分别从混合物中分离出两种主要的膜表面唾液糖肽（Y - SGP和O - SGP），产率约为45%和42%。Y - SGP和O - SGP的分子量分别为12,000和12,100。3. Y - SGP的唾液酸比例高于O - SGP。Y - SGP的半乳糖、N - 乙酰葡糖胺和N - 乙酰半乳糖胺含量显著低于O - SGP。4. 两种SGP对MN试剂表现出相同的反应性，但Y - SGP对鲎和菜豆（E）凝集素的反应性强于O - SGP。Y - SGP和O - SGP对花生（抗T）凝集素均无反应性。