University of Łódź, Department of General Biochemistry, Faculty of Biology and Environmental Protection, 90-236, Łódź, Poland.
Department of Biochemistry, Institute of Soil Science and Plant Cultivation, State Research Institute, 24-100, Puławy, Poland.
Biomed Pharmacother. 2021 May;137:111301. doi: 10.1016/j.biopha.2021.111301. Epub 2021 Feb 6.
Paulownia Clone in Vitro 112, also known as Oxytree is a hybrid of Paulownia elongata and Paulownia fortunei, developed under laboratory conditions. Its seeds are sterile, making it a noninvasive variety that can only be propagated in the laboratory. In China, species from the Paulownia genus (Paulowniaceae) are widely used in traditional medicine for the treatment of infectious diseases, such as gonorrhea and erysipelas. It has a broad spectrum of bioactivity, including neuroprotective, antioxidant, antibacterial, antiphlogistic, antiviral, and cytotoxic actions. However, the antiplatelet potential of Paulownia Clone in Vitro 112 has not yet been described.
The aim of our study was thus to examine the effect of an extract and four fractions from leaves of Paulownia Clone in Vitro 112 on various parameters of platelet activation in an in vitro model.
Composition of the investigated extract and fractions was determined by UHPLC-UV-MS. The following parameters of platelet activation were investigated: nonenzymatic lipid peroxidation in resting platelets; enzymatic lipid peroxidation (AA metabolism) in platelets activated by thrombin; superoxide anion (O) generation in the resting and activated platelets; platelet adhesion to collagen type I and fibrinogen; platelet aggregation stimulated by various physiological agonists, such as ADP, collagen, and thrombin. The effect of the extract and fractions on extracellular LDH activity, a marker of cell damage, was also determined.
Verbascoside a phenylethnanoid glycoside, was the main secondary metabolite of the extract from leaves of oxytree (constituting approximately 45 % of all compounds). There were also iridoids, such as catalpol, aucubin, and 7-hydroxytomentoside, as well as flavonoids, such as luteolin and apigenin glycosides. Moreover, the extract had stronger antiplatelet properties than the fractions. For example, the extract at 10 μg/mL inhibited five parameters of platelet activation.
Our results show that Paulownia Clone in Vitro 112 leaves are a new valuable source of compounds with antiplatelet potential.
“泡桐试管 112”又称“氧化树”，是泡桐属和泡桐属的杂交种，在实验室条件下培育而成。其种子不育，属于非入侵性品种，只能在实验室中繁殖。在中国，泡桐属（泡桐科）的物种被广泛用于治疗淋病和丹毒等传染病的传统医学。它具有广泛的生物活性，包括神经保护、抗氧化、抗菌、抗炎、抗病毒和细胞毒性作用。然而，“泡桐试管 112”的抗血小板潜力尚未被描述。
因此，我们的研究旨在检查泡桐试管 112 叶的提取物和四个馏分对体外模型中血小板激活的各种参数的影响。
通过 UHPLC-UV-MS 确定了被调查提取物和馏分的组成。研究了以下血小板激活参数：静止血小板中非酶脂质过氧化；凝血酶激活血小板中的酶促脂质过氧化（AA 代谢）；静止和激活血小板中超氧阴离子（O）的产生；血小板对胶原蛋白 I 和纤维蛋白原的黏附；各种生理激动剂（如 ADP、胶原蛋白和凝血酶）刺激的血小板聚集。还测定了提取物和馏分对细胞外 LDH 活性（细胞损伤的标志物）的影响。
苯丙素糖苷毛蕊花糖苷是氧化树叶片提取物中的主要次生代谢物（约占所有化合物的 45%）。还有环烯醚萜，如梓醇、梓醇和 7-羟基梓醇，以及黄酮类，如木犀草素和芹菜素糖苷。此外，提取物的抗血小板特性强于馏分。例如，提取物在 10μg/mL 时抑制了血小板激活的五个参数。
我们的结果表明，泡桐试管 112 叶是具有抗血小板潜力的化合物的新有价值来源。
